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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FCGR3A/CD16a Polyclonal antibody (30356-1-AP)

    WB analysis using 30356-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 30356-1-AP (FCGR3A/CD16a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using FCGR3A/CD16a Polyclonal antibody (30356-1-AP)

    WB analysis using 30356-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30356-1-AP (FCGR3A/CD16a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using FCGR3A/CD16a Polyclonal antibody (30356-1-AP)

    IF Staining of mouse spleen using 30356-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using FCGR3A/CD16a antibody (30356-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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FCGR3A/CD16a Polyclonal antibody

FCGR3A/CD16a Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 30356-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF-P, ELISA

Synonyms

CD16, Fc gamma RIII alpha, Fc gamma RIII, CD16-II, CD16a antigen

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHL-60 cells, Jurkat cells
Positive IF-P detected inmouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30356-1-AP targets FCGR3A/CD16a in WB, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FCGR3A/CD16a fusion protein Ag1281 相同性解析による交差性が予測される生物種
Full Name Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Calculated molecular weight 29 kDa
Observed molecular weight65-70 kDa
GenBank accession numberBC036723
Gene Symbol CD16a
Gene ID (NCBI) 2214
ENSEMBL Gene IDENSG00000203747
RRIDAB_3086300
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08637
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD16 is a low affinity Fc receptor. It has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. In humans, FcγRIIIa/CD16a is a type I transmembrane receptor expressed on macrophages and NK cells. FcγRIIIb/CD16b is a GPI-linked Fc-receptor highly expressed on neutrophils. The two proteins differ by only four amino acid residues in the mature proteins(PMID: 30361439). FCGR3A has a calculated molecular weight of 29 kDa, with an apparent molecular weight of 50-80 kDa due to extensive glycosylation (PMID: 30655272; PMID: 27914599). The antibody is specific for FCGR3A/CD16a.

Protocols

Product Specific Protocols
WB protocol for FCGR3A/CD16a antibody 30356-1-APDownload protocol
IF protocol for FCGR3A/CD16a antibody 30356-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols