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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FCGR3B/CD16b Polyclonal antibody

FCGR3B/CD16b Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30201-1-AP

Synonyms

CD16, CD16b, Fc gamma RIII, Fc gamma RIII beta, Fc gamma RIIIb, FCG3, FCGR3, FCGR3B, FcR 10, FcRIII, FcRIIIb, IGFR3, IgG Fc receptor III 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FCGR3B/CD16b Polyclonal antibody (30201-1-AP)

    WB analysis using 30201-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30201-1-AP (FCGR3B/CD16b antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using FCGR3B/CD16b Polyclonal antibody (30201-1-AP)

    IF Staining of A549 using 30201-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using FCGR3B/CD16b antibody (30201-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inRaji cells, U-937 cells, rat spleen tissue
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30201-1-AP targets FCGR3B/CD16b in WB, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen fusion protein 相同性解析による交差性が予測される生物種
Full Name Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
Calculated molecular weight26 kDa
Observed molecular weight55 kDa
GenBank accession numberNM_000570
Gene symbol FCGR3B
Gene ID (NCBI) 2215
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD16 is a low affinity Fc receptor. It has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and the FCGR3B. In humans, FcγRIIIa/CD16a is a type I transmembrane receptor expressed on macrophages and NK cells. FcγRIIIb/CD16b is a GPI-linked Fc-receptor highly expressed on neutrophils. The two proteins differ by only four amino acid residues in the mature proteins(PMID: 30361439). FCGR3B/CD16b can be detected as 26 kDa (PMID: 34108956; 36096917) and 50-75 kDa (PMID: 30655272; 18208407). The antibody is specific for FCGR3B/CD16b.

Protocols

Product Specific Protocols
WB protocol for FCGR3B/CD16b antibody 30201-1-APDownload protocol
IF protocol for FCGR3B/CD16b antibody 30201-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols