FCHSD1 Polyclonal antibody

Name: FCHSD1 Polyclonal antibody
Brand: Proteintech
SKU: 23362-1-AP

FCHSD1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 23362-1-AP

Synonyms

FCH and double SH3 domains 1, FCHSD1, NWK2

Validation Data Gallery

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AllWBIHCIF-P

WB analysis using 23362-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 23362-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 23362-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 23362-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 23362-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 23362-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 23362-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 23362-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 23362-1-AP (FCHSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 23362-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23362-1-AP (FCHSD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 23362-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23362-1-AP (FCHSD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse kidney using 23362-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using FCHSD1 antibody (23362-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 23362-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, K-562 cells, HeLa cells, Jurkat cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse kidney tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below

Product Information

23362-1-AP targets FCHSD1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	FCHSD1 fusion protein Ag19963 相同性解析による交差性が予測される生物種
Full Name	FCH and double SH3 domains 1
Calculated molecular weight	690 aa, 77 kDa
Observed molecular weight	46-50 kDa
GenBank accession number	BC047016
Gene symbol	FCHSD1
Gene ID (NCBI)	89848
RRID	AB_2879265
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

FCHSD1 (FCH and double SH3 domains 1), also known as NWK2, is a member of the F-BAR family, which is involved in endocytosis and vesicle trafficking. FCHSD1 is expressed in mouse cochlear sensory hair cells and plays an important role in regulating the assembly or maintenance of F-actin in hair cell stereocilia and cuticular plate (PMID: 23437151). FCHSD1 also plays a role in stress response sensing and oxidative stress integration and is involved in the pathogenesis of emphysema (PMID: 34168078). FCHSD1 has three isoforms with molecular weights of 77 kDa, 46 kDa, and 40 kDa.

Protocols

Product Specific Protocols
WB protocol for FCHSD1 antibody 23362-1-AP	Download protocol
IHC protocol for FCHSD1 antibody 23362-1-AP	Download protocol
IF protocol for FCHSD1 antibody 23362-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A Loss of FCHSD1 leads to amelioration of chronic obstructive pulmonary disease. Authors - Takahiro Kawasaki View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FCHSD1 Polyclonal antibody

FCHSD1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Validation Data Gallery

WB analysis using 23362-1-AP

WB analysis of HEK-293 using 23362-1-AP

WB analysis of Jurkat using 23362-1-AP

WB analysis of HEK-293 using 23362-1-AP

WB analysis of K-562 using 23362-1-AP

WB analysis of HeLa using 23362-1-AP