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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FDFT1 Recombinant antibody (83020-1-RR)

    WB analysis using 83020-1-RR (same clone as 83020-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83020-1-RR (FDFT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83020-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using FDFT1 Recombinant antibody (83020-1-RR)

    IHC staining of human ovary cancer using 83020-1-RR (same clone as 83020-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83020-1-RR (FDFT1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83020-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FDFT1 Recombinant antibody (83020-1-RR)

    IF Staining of HepG2 using 83020-1-RR (same clone as 83020-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FDFT1 antibody (83020-1-RR, Clone: 230362C10 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83020-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using FDFT1 Recombinant antibody (83020-1-RR)

    IF Staining of L02 using 83020-1-RR (same clone as 83020-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed L02 cells using FDFT1 antibody (83020-1-RR, Clone: 230362C10 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83020-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using FDFT1 Recombinant antibody (83020-1-RR)

    FC experiment of U2OS using 83020-1-RR (same clone as 83020-1-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human FDFT1 (83020-1-RR, Clone:230362C10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83020-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83020-1-RR

    Affinity and Kinetic Characterization of 83020-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83020-1-RR against Human FDFT1 were performed. The affinity constant is 0.126 nM.

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FDFT1 Recombinant antibody, PBS Only

FDFT1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83020-1-PBS
Clone No.230362C10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Farnesyl-diphosphate farnesyltransferase 1, Farnesyl-diphosphate farnesyltransferase, ERG9, EC:2.5.1.21, DGPT

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83020-1-PBS targets FDFT1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FDFT1 fusion protein Ag3743 相同性解析による交差性が予測される生物種
Full Name farnesyl-diphosphate farnesyltransferase 1
Calculated molecular weight 417 aa, 48 kDa
Observed molecular weight48 kDa
GenBank accession numberBC029641
Gene Symbol FDFT1
Gene ID (NCBI) 2222
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP37268
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

SQS (Squalene synthase), also known as Farnesyl-diphosphate farnesyltransferase 1 (FDFT1) is a gene that encodes the membrane-associated enzyme squalene synthase, which is the first specific enzyme in cholesterol biosynthesis. FDFT1 is highly expressed in liver, lung, prostate, breast, ovary, bladder, cervix, thyroid, and esophageal cancers, while in colorectal, colon, testicular, uterine, pancreas, and kidney tumors, its expression is downregulated (PMID: 35093030). FDFT1 is a key downstream target of the fasting response and may be involved in CRC cell glucose metabolism (PMID: 32313017).