Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue, pig adrenal gland tissue, human adrenal gland tissue, rat testis tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
Product Information
15584-1-AP targets FDXR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FDXR fusion protein Ag7145 相同性解析による交差性が予測される生物種 |
| Full Name | ferredoxin reductase |
| Calculated molecular weight | 54 kDa |
| Observed molecular weight | 48-58 kDa |
| GenBank accession number | BC063493 |
| Gene Symbol | FDXR |
| Gene ID (NCBI) | 2232 |
| RRID | AB_2102602 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P22570 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ferredoxin reductase (FDXR, also known as adrenodoxin reductase) is a mitochondrial membrane-associated flavoprotein. One of its functions is to transfer electrons from NADPH to the two human ferredoxin proteins, FDX1 and FDX2. FDXR is expressed in all tissues with the highest expression in tissues such as the adrenal cortex, that specialize in steroid hormone synthesis. Functionally, FDXR is suggested to be involved in various Functionally, FDXR is suggested to be involved in various. Notably, recent studies have shown that FDXR mutations are associated with mitochondrial disorders, probably due to its role in iron-sulfur cluster protein biosynthesis (PMID: 32304229). FDXR has 7 isoforms with the molecular weight of 48-58 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FDXR antibody 15584-1-AP | Download protocol |
| IHC protocol for FDXR antibody 15584-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Biol Chem The neurosteroid pregnenolone is synthesized by a mitochondrial P450 enzyme other than CYP11A1 in human glial cells. | ||
Front Oncol FDXR drives primary and endocrine-resistant tumor cell growth in ER+ breast cancer via CPT1A-mediated fatty acid oxidation
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