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Validation Data Gallery

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  • Western Blot (WB) analysis of U-937 cells using FEM1A Polyclonal antibody (15169-1-AP)

    WB analysis of U-937 using 15169-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 15169-1-AP (FEM1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using FEM1A Polyclonal antibody (15169-1-AP)

    IHC staining of human skeletal muscle using 15169-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15169-1-AP (FEM1A antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using FEM1A Polyclonal antibody (15169-1-AP)

    IHC staining of human skeletal muscle using 15169-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15169-1-AP (FEM1A antibody) at dilution of 1:50 (under 40x lens).

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FEM1A Polyclonal antibody

FEM1A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15169-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

EPRAP, fem 1 homolog a (C. elegans), FEM1 alpha, FEM1A, Protein fem 1 homolog A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU-937 cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15169-1-AP targets FEM1A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FEM1A fusion protein Ag7357 相同性解析による交差性が予測される生物種
Full Name fem-1 homolog a (C. elegans)
Calculated molecular weight 74 kDa
Observed molecular weight 74 kDa
GenBank accession numberBC004988
Gene Symbol FEM1A
Gene ID (NCBI) 55527
RRIDAB_2102618
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BSK4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FEM1A, also named as Protein fem-1 homolog A-A, is a 654 amino acid protein, which Belongs to the fem-1 family. FEM1A is highly expressed in adult heart and skeletal muscle. FEM1A is a Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. FEM1A may participate in antiinflammatory signaling via its interaction with PTGER4.Furthermore, Fem1aexpression is down-regulated in rhabdomyosarcoma cells suggesting that the FEM1A gene may be involved in muscle differentiation or be at least a marker of abnormal differentiation in rhabdomyosarcoma. Knowing that a molecular weight of 76 kDa is predicted for human FEM1A, but phosphorylated FEM1A is 95-100 kDa (PMID: 19406122).

Protocols

Product Specific Protocols
WB protocol for FEM1A antibody 15169-1-APDownload protocol
IHC protocol for FEM1A antibody 15169-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols