Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, mouse testis tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
11030-1-AP targets FEM1B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FEM1B fusion protein Ag1497 相同性解析による交差性が予測される生物種 |
| Full Name | fem-1 homolog b (C. elegans) |
| Calculated molecular weight | 70 kDa |
| Observed molecular weight | 61 kDa |
| GenBank accession number | BC010122 |
| Gene Symbol | FEM1B |
| Gene ID (NCBI) | 10116 |
| RRID | AB_2102637 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UK73 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FEM1B, also named F1AA, and KIAA0396 is a 627 amino acid protein belonging to the fem-1 family. FEM1B is highly expressed in the testis and weakly expressed in other tissues. FEM1B localizes both in the nucleus and cytoplasm. In the nucleus, the protein level increased slightly after camptothecin (CPT) treatment. FEM1B is a component of an E3 ubiquitin-protein ligase complex, which may act as a substrate recognition subunit. FEM1B is involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. FEM1B is also involved in glucose homeostasis in pancreatic islets. FEM1B functions as an adapter/mediator in replication stress-induced signaling that leads to the activation of CHEK1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FEM1B antibody 11030-1-AP | Download protocol |
| IHC protocol for FEM1B antibody 11030-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Reactive oxygen species control protein degradation at the mitochondrial import gate |