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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FEM1B Polyclonal antibody

FEM1B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11030-1-AP

Synonyms

F1A alpha, F1AA, fem 1 homolog b (C. elegans), FEM1 beta, FEM1B, FIAA, KIAA0396, Protein fem 1 homolog B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FEM1B Polyclonal antibody (11030-1-AP)

    WB analysis using 11030-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11030-1-AP (FEM1B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using FEM1B Polyclonal antibody (11030-1-AP)

    IHC staining of human prostate cancer using 11030-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11030-1-AP (FEM1B antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

Positive WB detected inJurkat cells, mouse testis tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11030-1-AP targets FEM1B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FEM1B fusion protein Ag1497 相同性解析による交差性が予測される生物種
Full Name fem-1 homolog b (C. elegans)
Calculated molecular weight 70 kDa
Observed molecular weight61 kDa
GenBank accession numberBC010122
Gene symbol FEM1B
Gene ID (NCBI) 10116
RRIDAB_2102637
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FEM1B, also named F1AA, and KIAA0396 is a 627 amino acid protein, which belongs to the fem-1 family. FEM1B is highly expressed in the testis and weakly expressed in other tissues. FEM1B localizes both in the nucleus and cytoplasm. In the nucleus, the protein level increased slightly after camptothecin (CPT) treatment. FEM1B is a component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit. FEM1B is involved in apoptosis by acting as a death receptor-associated protein that mediates apoptosis. FEM1B is also involved in glucose homeostasis in pancreatic islets. FEM1B functions as an adapter/mediator in replication stress-induced signaling that leads to the activation of CHEK1.

Protocols

Product Specific Protocols
WB protocol for FEM1B antibody 11030-1-APDownload protocol
IHC protocol for FEM1B antibody 11030-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols