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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FEM1C Polyclonal antibody (14037-1-AP)

    WB analysis using 14037-1-AP (same clone as 14037-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using FEM1C Polyclonal antibody (14037-1-AP)

    WB analysis of mouse brain using 14037-1-AP (same clone as 14037-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using FEM1C Polyclonal antibody (14037-1-AP)

    WB analysis of mouse brain using 14037-1-AP (same clone as 14037-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using FEM1C Polyclonal antibody (14037-1-AP)

    WB analysis of mouse heart using 14037-1-AP (same clone as 14037-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using FEM1C Polyclonal antibody (14037-1-AP)

    WB analysis of mouse heart using 14037-1-AP (same clone as 14037-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using FEM1C Polyclonal antibody (14037-1-AP)

    WB analysis of HepG2 using 14037-1-AP (same clone as 14037-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using FEM1C Polyclonal antibody (14037-1-AP)

    WB analysis of HepG2 using 14037-1-AP (same clone as 14037-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using FEM1C Polyclonal antibody (14037-1-AP)

    IHC staining of human heart using 14037-1-AP (same clone as 14037-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14037-1-AP (FEM1C Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FEM1C Polyclonal antibody (14037-1-AP)

    IHC staining of mouse brain using 14037-1-AP (same clone as 14037-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14037-1-AP (FEM1C Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FEM1C Polyclonal antibody (14037-1-AP)

    IHC staining of mouse brain using 14037-1-AP (same clone as 14037-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14037-1-AP (FEM1C Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using FEM1C Polyclonal antibody (14037-1-AP)

    IHC staining of human heart using 14037-1-AP (same clone as 14037-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14037-1-AP (FEM1C Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FEM1C Polyclonal antibody (14037-1-AP)

    IF Staining of HeLa using 14037-1-AP (same clone as 14037-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FEM1C antibody (14037-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

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FEM1C Polyclonal antibody, PBS Only

FEM1C Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 14037-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14037-1-PBS targets FEM1C in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FEM1C fusion protein Ag5099 相同性解析による交差性が予測される生物種
Full Name fem-1 homolog c (C. elegans)
Calculated molecular weight 69 kDa
Observed molecular weight 69 kDa
GenBank accession numberBC028369
Gene Symbol FEM1C
Gene ID (NCBI) 56929
RRIDAB_2878002
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96JP0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Protein fem-1 homolog C (FEM1C) is involved in protein modification and ubiquitination.Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit.Highly expressed in kidney, cardiac tissue, skeletal muscle and testis. Expressed at lower levels in other tissues, including cartilage.This antibody specifically recognizes the 69kd FEM1C protein.