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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FEM1C Polyclonal antibody, PBS Only

FEM1C Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14037-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using FEM1C Polyclonal antibody (14037-1-AP)

    WB analysis using 14037-1-AP (same clone as 14037-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using FEM1C Polyclonal antibody (14037-1-AP)

    WB analysis of mouse brain using 14037-1-AP (same clone as 14037-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using FEM1C Polyclonal antibody (14037-1-AP)

    WB analysis of mouse brain using 14037-1-AP (same clone as 14037-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using FEM1C Polyclonal antibody (14037-1-AP)

    WB analysis of mouse heart using 14037-1-AP (same clone as 14037-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse heart tissue using FEM1C Polyclonal antibody (14037-1-AP)

    WB analysis of mouse heart using 14037-1-AP (same clone as 14037-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using FEM1C Polyclonal antibody (14037-1-AP)

    WB analysis of HepG2 using 14037-1-AP (same clone as 14037-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using FEM1C Polyclonal antibody (14037-1-AP)

    WB analysis of HepG2 using 14037-1-AP (same clone as 14037-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14037-1-AP (FEM1C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using FEM1C Polyclonal antibody (14037-1-AP)

    IHC staining of human heart using 14037-1-AP (same clone as 14037-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14037-1-AP (FEM1C Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using FEM1C Polyclonal antibody (14037-1-AP)

    IHC staining of human heart using 14037-1-AP (same clone as 14037-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14037-1-AP (FEM1C Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FEM1C Polyclonal antibody (14037-1-AP)

    IHC staining of mouse brain using 14037-1-AP (same clone as 14037-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14037-1-AP (FEM1C Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FEM1C Polyclonal antibody (14037-1-AP)

    IHC staining of mouse brain using 14037-1-AP (same clone as 14037-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14037-1-AP (FEM1C Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FEM1C Polyclonal antibody (14037-1-AP)

    IF Staining of HeLa using 14037-1-AP (same clone as 14037-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FEM1C antibody (14037-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14037-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14037-1-PBS targets FEM1C in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FEM1C fusion protein Ag5099 相同性解析による交差性が予測される生物種
Full Name fem-1 homolog c (C. elegans)
Calculated molecular weight 69 kDa
Observed molecular weight 69 kDa
GenBank accession numberBC028369
Gene symbol FEM1C
Gene ID (NCBI) 56929
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Protein fem-1 homolog C (FEM1C) is involved in protein modification and ubiquitination.Probable component of an E3 ubiquitin-protein ligase complex, in which it may act as a substrate recognition subunit.Highly expressed in kidney, cardiac tissue, skeletal muscle and testis. Expressed at lower levels in other tissues, including cartilage.This antibody specifically recognizes the 69kd FEM1C protein.