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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FER Polyclonal antibody, PBS Only

FER Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 25287-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of NIH/3T3 cells using FER Polyclonal antibody (25287-1-AP)

    WB analysis of NIH/3T3 using 25287-1-AP (same clone as 25287-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25287-1-AP (FER Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25287-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using FER Polyclonal antibody (25287-1-AP)

    WB analysis of MCF-7 using 25287-1-AP (same clone as 25287-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25287-1-AP (FER Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25287-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using FER Polyclonal antibody (25287-1-AP)

    WB analysis of HeLa using 25287-1-AP (same clone as 25287-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 25287-1-AP (FER Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25287-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using FER Polyclonal antibody (25287-1-AP)

    WB analysis of A431 using 25287-1-AP (same clone as 25287-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 25287-1-AP (FER Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25287-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using FER Polyclonal antibody (25287-1-AP)

    IP experiment of MCF-7 using 25287-1-AP (same clone as 25287-1-PBS)

    IP result of anti-FER (IP:25287-1-AP, 4ug; Detection:25287-1-AP 1:1000) with MCF-7 cells lysate 2400ug. This data was developed using the same antibody clone with 25287-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using FER Polyclonal antibody (25287-1-AP)

    IHC staining of human kidney using 25287-1-AP (same clone as 25287-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25287-1-AP (FER antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25287-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FER Polyclonal antibody (25287-1-AP)

    IHC staining of human colon cancer using 25287-1-AP (same clone as 25287-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25287-1-AP (FER Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25287-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FER Polyclonal antibody (25287-1-AP)

    IHC staining of human colon cancer using 25287-1-AP (same clone as 25287-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25287-1-AP (FER Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25287-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using FER Polyclonal antibody (25287-1-AP)

    IHC staining of human kidney using 25287-1-AP (same clone as 25287-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25287-1-AP (FER antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25287-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using FER Polyclonal antibody (25287-1-AP)

    IF Staining of MCF-7 using 25287-1-AP (same clone as 25287-1-PBS)

    Immunofluorescent analysis of MCF-7 cells using 25287-1-AP (FER antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25287-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using FER Polyclonal antibody (25287-1-AP)

    IF Staining of MCF-7 using 25287-1-AP (same clone as 25287-1-PBS)

    Immunofluorescent analysis of MCF-7 cells using 25287-1-AP (FER antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25287-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25287-1-PBS targets FER in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FER fusion protein Ag18173 相同性解析による交差性が予測される生物種
Full Name fer (fps/fes related) tyrosine kinase
Calculated molecular weight 822 aa, 95 kDa
Observed molecular weight 95 kDa
GenBank accession numberBC017060
Gene symbol FER
Gene ID (NCBI) 2241
RRIDAB_2880009
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

FER(Tyrosine-protein kinase Fer) is also named as TYK3 and belongs to the protein kinase superfamily. Tyrosine-protein kinase acts downstream of cell surface receptors for growth factors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. This protein has 3 isoforms produced by alternative promoter usage and alternative splicing.