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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FES Polyclonal antibody

FES Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28194-1-AP

Synonyms

feline sarcoma oncogene, FES, FPS, Proto oncogene c Fes, Proto oncogene c Fps

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FES Polyclonal antibody (28194-1-AP)

    WB analysis using 28194-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28194-1-AP (FES antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FES Polyclonal antibody (28194-1-AP)

    IHC staining of human colon cancer using 28194-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28194-1-AP (FES antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FES Polyclonal antibody (28194-1-AP)

    IHC staining of human colon cancer using 28194-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28194-1-AP (FES antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FES Polyclonal antibody (28194-1-AP)

    IHC staining of human colon cancer using 28194-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28194-1-AP (FES antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FES Polyclonal antibody (28194-1-AP)

    IHC staining of human colon cancer using 28194-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28194-1-AP (FES antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHL-60 cells, THP-1 cells, U-937 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28194-1-AP targets FES in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FES fusion protein Ag28095 相同性解析による交差性が予測される生物種
Full Name feline sarcoma oncogene
Calculated molecular weight 822 aa, 94 kDa
Observed molecular weight 93 kDa, 79 kDa, 87 kDa, 85 kDa
GenBank accession numberBC035357
Gene symbol FES
Gene ID (NCBI) 2242
RRIDAB_2881086
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Feline sarcoma oncogene protein (FES) is a member of the FES/FES-related (FER) subfamily of non-receptor protein tyrosine kinases. FES was originally identified a retroviral oncogene in avian and feline retroviruses. Articles reported that FES plays a role in growth and differentiation of myeloid hematopoietic cells, vascular endothelial cells and neurons. And it also involved in the regulation of cytoskeletal rearrangement (PMID:15929003). Addition, a recent study suggests that FES may function both tumorigenic and tumor-suppressive effects (PMID: 28952025).

Protocols

Product Specific Protocols
WB protocol for FES antibody 28194-1-APDownload protocol
IHC protocol for FES antibody 28194-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols