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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FES Monoclonal antibody (67524-1-Ig)

    WB analysis using 67524-1-Ig (same clone as 67524-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67524-1-Ig (FES antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67524-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NR8383 cells using FES Monoclonal antibody (67524-1-Ig)

    WB analysis of NR8383 using 67524-1-Ig (same clone as 67524-1-PBS)

    NR8383 cells were subjected to SDS PAGE followed by western blot with 67524-1-Ig (FES antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67524-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Karpas-299 cells using FES Monoclonal antibody (67524-1-Ig)

    WB analysis of Karpas-299 using 67524-1-Ig (same clone as 67524-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67524-1-Ig (FES antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67524-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using FES Monoclonal antibody (67524-1-Ig)

    IF Staining of A549 using 67524-1-Ig (same clone as 67524-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using 67524-1-Ig (FES antibody), at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67524-1-PBS in a different storage buffer formulation.

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FES Monoclonal antibody, PBS Only

FES Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67524-1-PBS
Clone No.1E1B11

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Proto oncogene c Fes, p93c-fes, FPS, Feline sarcoma/Fujinami avian sarcoma oncogene homolog, EC:2.7.10.2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67524-1-PBS targets FES in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen FES fusion protein Ag28108 相同性解析による交差性が予測される生物種
Full Name feline sarcoma oncogene
Calculated molecular weight 822 aa, 94 kDa
Observed molecular weight 93 kDa, 79 kDa, 87 kDa, 85 kDa
GenBank accession numberBC035357
Gene Symbol FES
Gene ID (NCBI) 2242
RRIDAB_2882744
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07332
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.