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Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using FEV Polyclonal antibody (25058-1-AP)

    WB analysis of fetal human brain using 25058-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 25058-1-AP (FEV Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using FEV Polyclonal antibody (25058-1-AP)

    WB analysis of mouse brain using 25058-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25058-1-AP (FEV antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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FEV Polyclonal antibody

FEV Polyclonal Antibody for WB, ELISA
Cat No. 25058-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

PET 1, PC12 ETS factor 1, PC12 ETS domain-containing transcription factor 1, HSRNAFEV, Fifth Ewing variant protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected infetal human brain tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

25058-1-AP targets FEV in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FEV fusion protein Ag20647 相同性解析による交差性が予測される生物種
Full Name FEV (ETS oncogene family)
Calculated molecular weight 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC023511
Gene Symbol FEV
Gene ID (NCBI) 54738
RRIDAB_2879877
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99581
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FEV belongs to the ETS transcription factor family. ETS family members have a highly conserved 85-amino acid ETS domain that binds purine-rich DNA sequences. The alanine-rich C-terminus of this gene indicates that it may act as a transcription repressor. This gene is exclusively expressed in neurons of the central serotonin (5-HT) system, a system implicated in the pathogeny of such psychiatric diseases as depression, anxiety, and eating disorders. In some types of Ewing tumors, this gene is fused to the Ewing sarcoma (EWS) gene following chromosome translocations.

Protocols

Product Specific Protocols
WB protocol for FEV antibody 25058-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
View Article