• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FEZ2 Recombinant antibody

FEZ2 Recombinant Antibody for FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240248F5

Cat no : 83407-6-RR

Synonyms

Zygin-2, Zygin II, Zygin 2, HUM3CL, Fasciculation and elongation protein zeta-2

Validation Data Gallery

Filter:
  • Flow cytometry (FC) experiment of A431 cells using FEZ2 Recombinant antibody (83407-6-RR)

    FC experiment of A431 using 83407-6-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Fez2 Recombinant Antibody (83407-6-RR, Clone:240248F5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83407-6-RR

    Affinity and Kinetic Characterization of 83407-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83407-6-RR against Human FEZ2 were performed. The affinity constant is 6.64 nM.

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83407-6-RR is FEZ2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FEZ2 fusion protein Ag15370 相同性解析による交差性が予測される生物種
Full Name fasciculation and elongation protein zeta 2 (zygin II)
Calculated molecular weight 353 aa, 40 kDa
GenBank accession numberBC110386
Gene symbol FEZ2
Gene ID (NCBI) 9637
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FEZ2 is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles.

Protocols

Product Specific Protocols
FC protocol for FEZ2 antibody 83407-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols