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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FFAR2 Recombinant antibody (84544-1-RR)

    WB analysis using 84544-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84544-1-RR (FFAR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using FFAR2 Recombinant antibody (84544-1-RR)

    IF Staining of mouse brain using 84544-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using FFAR2 antibody (84544-1-RR, Clone: 241696B4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

FFAR2 Recombinant antibody

FFAR2 Recombinant Antibody for WB, IF-P, ELISA
Cat No. 84544-1-RR
Clone No.241696B4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Synonyms

GPCR43, G protein coupled receptor 43, free fatty acid receptor 2, FFA2R, FFA2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inTHP-1 cells, HCT 116 cells, HT-29 cells
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84544-1-RR targets FFAR2 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name free fatty acid receptor 2
Calculated molecular weight 330 aa, 37 kDa
Observed molecular weight37 kDa
GenBank accession numberBC096198
Gene Symbol FFAR2
Gene ID (NCBI) 2867
RRIDAB_3672049
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO15552
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Free fatty acid receptors (FFAR) play significant roles in various physiological processes through interaction with their ligands, fatty acids. Free fatty acid receptor 2 (FFAR2, also known as FFA2 or GPR43) is a receptor for short-chain fatty acids (SCFAs) and plays a role in the regulation of whole-body energy homeostasis and intestinal immunity (PMID: 12684041). It has been considered a therapeutic target for metabolic and inflammatory conditions (PMID: 23589301). FFAR2 has a calculated molecular weight of 37 kDa and can be glycosylated. The higher apparent molecular weight of 50 kDa has been reported, probably due to glycosylation (PMID: 31707282; 28131568).

Protocols

Product Specific Protocols
WB protocol for FFAR2 antibody 84544-1-RRDownload protocol
IF protocol for FFAR2 antibody 84544-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols