FFAR3 Monoclonal antibody
FFAR3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (3)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1D10B7
Cat no : 66811-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HepG2 cells, human adipose tissue, HT-29 cells |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A549 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 5 publications below |
IF | See 3 publications below |
Product Information
66811-1-Ig targets FFAR3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Cited Reactivity | human, rat, pig |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | FFAR3 fusion protein Ag28025 相同性解析による交差性が予測される生物種 |
Full Name | free fatty acid receptor 3 |
Calculated molecular weight | 39 kDa |
Observed molecular weight | 40-48 kDa |
GenBank accession number | BC035657 |
Gene symbol | FFAR3 |
Gene ID (NCBI) | 2865 |
RRID | AB_2882154 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FFAR3 (Free fatty acid receptors 3) also known as GPR41, with another GPR43 protein, both are Gai-coupled receptor activated by short-chain fatty acids (SCFAs) such as acetate, propionate, and butyrate (PMID:26870043). GPR41 protein is translated from the bicistronic mRNA encoding GPR40 and GPR41, where an internal ribosome entry site (IRES) is utilized for the GPR41 coding sequence downstream of GPR40 (PMID:22493486). GPR41 is expressed in adipose tissue, gut, and the peripheral nervous system, and it is involved in SCFA-dependent energy regulation(PMID: 24904531).
Protocols
Product Specific Protocols | |
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WB protocol for FFAR3 antibody 66811-1-Ig | Download protocol |
IHC protocol for FFAR3 antibody 66811-1-Ig | Download protocol |
IF protocol for FFAR3 antibody 66811-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Front Immunol Natural fish oil improves the differentiation and maturation of oligodendrocyte precursor cells to oligodendrocytes in vitro after interaction with the blood-brain barrier | ||
Front Microbiol Gut microbiome and serum short-chain fatty acids are associated with responses to chemo- or targeted therapies in Chinese patients with lung cancer | ||
Gut Microbes Butyrate reduces adherent-invasive E. coli-evoked disruption of epithelial mitochondrial morphology and barrier function: involvement of free fatty acid receptor 3 | ||
Gut Microbes Sialic acid-based probiotic intervention in lactating mothers improves the neonatal gut microbiota and immune responses by regulating sialylated milk oligosaccharide synthesis via the gut-breast axis | ||
Food Funct Improving insulin resistance by sulforaphane via activating the Bacteroides and Lactobacillus SCFAs-GPR-GLP1 signal axis | ||
PLoS One Modeling of culture conditions by culture system, glucose and propionic acid and their impact on metabolic profile in IPEC-J2 |