FFAR3 Monoclonal antibody

FFAR3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (3)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D10B7

Cat no : 66811-1-Ig

Synonyms

FFA3R, FFAR3, free fatty acid receptor 3, G protein coupled receptor 41, GPR41


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Tested Applications

Positive WB detected inHepG2 cells, human adipose tissue, HT-29 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66811-1-Ig targets FFAR3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FFAR3 fusion protein Ag28025 相同性解析による交差性が予測される生物種
Full Name free fatty acid receptor 3
Calculated molecular weight 39 kDa
Observed molecular weight 40-48 kDa
GenBank accession numberBC035657
Gene symbol FFAR3
Gene ID (NCBI) 2865
RRIDAB_2882154
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FFAR3 (Free fatty acid receptors 3) also known as GPR41, with another GPR43 protein, both are Gai-coupled receptor activated by short-chain fatty acids (SCFAs) such as acetate, propionate, and butyrate (PMID:26870043). GPR41 protein is translated from the bicistronic mRNA encoding GPR40 and GPR41, where an internal ribosome entry site (IRES) is utilized for the GPR41 coding sequence downstream of GPR40 (PMID:22493486). GPR41 is expressed in adipose tissue, gut, and the peripheral nervous system, and it is involved in SCFA-dependent energy regulation(PMID: 24904531).

Protocols

Product Specific Protocols
WB protocol for FFAR3 antibody 66811-1-IgDownload protocol
IHC protocol for FFAR3 antibody 66811-1-IgDownload protocol
IF protocol for FFAR3 antibody 66811-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Front Immunol

Natural fish oil improves the differentiation and maturation of oligodendrocyte precursor cells to oligodendrocytes in vitro after interaction with the blood-brain barrier

Authors - Paweł Piatek
humanWB,IF

Front Microbiol

Gut microbiome and serum short-chain fatty acids are associated with responses to chemo- or targeted therapies in Chinese patients with lung cancer

Authors - Huan-Huan Chen
humanWB

Gut Microbes

Butyrate reduces adherent-invasive E. coli-evoked disruption of epithelial mitochondrial morphology and barrier function: involvement of free fatty acid receptor 3

Authors - Samira A Hamed
humanWB

Gut Microbes

Sialic acid-based probiotic intervention in lactating mothers improves the neonatal gut microbiota and immune responses by regulating sialylated milk oligosaccharide synthesis via the gut-breast axis

Authors - Yushuang Wang
ratWB

Food Funct

Improving insulin resistance by sulforaphane via activating the Bacteroides and Lactobacillus SCFAs-GPR-GLP1 signal axis

Authors - Sicong Tian
pigIF

PLoS One

Modeling of culture conditions by culture system, glucose and propionic acid and their impact on metabolic profile in IPEC-J2

Authors - Shirko Marcel Shokr