FGD1 Polyclonal antibody
FGD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 13141-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | BxPC-3 cells, SK-N-SH cells, U2OS cells |
Positive IP detected in | SK-N-SH cells |
Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | U2OS cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
13141-1-AP targets FGD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | FGD1 fusion protein Ag3861 相同性解析による交差性が予測される生物種 |
Full Name | FYVE, RhoGEF and PH domain containing 1 |
Calculated molecular weight | 901 aa, 91 kDa |
Observed molecular weight | 130 kDa |
GenBank accession number | BC034530 |
Gene symbol | FGD1 |
Gene ID (NCBI) | 2245 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FGD1 is also named as FGDY (Faciogenital dysplasia 1 protein), ZFYVE3 (Zinc finger FYVE domain-containing protein 3), Rho/Rac guanine nucleotide exchange factor FGD1 (Rho/Rac GEF), FYVE, RhoGEF and PH domain-containing protein 1. FGD1 activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. And FGD1 plays a role in regulating the actin cytoskeleton and cell shape. (PMID: 8969170). FGD1 was markedly overexpressed and might be a prognostic biomarker in osteosarcoma patient specimens (PMID: 32194840). FGD1 is specific for the Rho GTPase cell division cycle 42 (CDC42) (PMID: 22854039).
Protocols
Product Specific Protocols | |
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WB protocol for FGD1 antibody 13141-1-AP | Download protocol |
IHC protocol for FGD1 antibody 13141-1-AP | Download protocol |
IF protocol for FGD1 antibody 13141-1-AP | Download protocol |
IP protocol for FGD1 antibody 13141-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |