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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FGD1 Polyclonal antibody

FGD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13141-1-AP

Synonyms

Zinc finger FYVE domain-containing protein 3, ZFYVE3, Rho/Rac GEF, FYVE, RhoGEF and PH domain-containing protein 1, FGDY

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FGD1 Polyclonal antibody (13141-1-AP)

    WB analysis using 13141-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13141-1-AP (FGD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using FGD1 Polyclonal antibody (13141-1-AP)

    WB analysis using 13141-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13141-1-AP (FGD1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of SK-N-SH cells using FGD1 Polyclonal antibody (13141-1-AP)

    IP experiment of SK-N-SH using 13141-1-AP

    IP result of anti-FGD1 (IP:13141-1-AP, 4ug; Detection:13141-1-AP 1:600) with SK-N-SH cells lysate 1160 ug.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using FGD1 Polyclonal antibody (13141-1-AP)

    IHC staining of mouse kidney using 13141-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13141-1-AP (FGD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using FGD1 Polyclonal antibody (13141-1-AP)

    IF Staining of U2OS using 13141-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using FGD1 antibody (13141-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

Positive WB detected inBxPC-3 cells, SK-N-SH cells, U2OS cells
Positive IP detected inSK-N-SH cells
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13141-1-AP targets FGD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FGD1 fusion protein Ag3861 相同性解析による交差性が予測される生物種
Full Name FYVE, RhoGEF and PH domain containing 1
Calculated molecular weight 901 aa, 91 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC034530
Gene symbol FGD1
Gene ID (NCBI) 2245
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FGD1 is also named as FGDY (Faciogenital dysplasia 1 protein), ZFYVE3 (Zinc finger FYVE domain-containing protein 3), Rho/Rac guanine nucleotide exchange factor FGD1 (Rho/Rac GEF), FYVE, RhoGEF and PH domain-containing protein 1. FGD1 activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. And FGD1 plays a role in regulating the actin cytoskeleton and cell shape. (PMID: 8969170). FGD1 was markedly overexpressed and might be a prognostic biomarker in osteosarcoma patient specimens (PMID: 32194840). FGD1 is specific for the Rho GTPase cell division cycle 42 (CDC42) (PMID: 22854039).

Protocols

Product Specific Protocols
WB protocol for FGD1 antibody 13141-1-APDownload protocol
IHC protocol for FGD1 antibody 13141-1-APDownload protocol
IF protocol for FGD1 antibody 13141-1-APDownload protocol
IP protocol for FGD1 antibody 13141-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols