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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FGD2 Polyclonal antibody

FGD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27068-1-AP

Synonyms

Zinc finger FYVE domain-containing protein 4, ZFYVE4, FYVE, RhoGEF and PH domain-containing protein 2

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using FGD2 Polyclonal antibody (27068-1-AP)

    WB analysis of Raji using 27068-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 27068-1-AP (FGD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using FGD2 Polyclonal antibody (27068-1-AP)

    IHC staining of human tonsillitis using 27068-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27068-1-AP (FGD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using FGD2 Polyclonal antibody (27068-1-AP)

    IHC staining of human tonsillitis using 27068-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 27068-1-AP (FGD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using FGD2 Polyclonal antibody (27068-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 27068-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27068-1-AP (FGD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using FGD2 Polyclonal antibody (27068-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 27068-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 27068-1-AP (FGD2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inRaji cells
Positive IHC detected inhuman tonsillitis tissue, human intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27068-1-AP targets FGD2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FGD2 fusion protein Ag25782 相同性解析による交差性が予測される生物種
Full Name FYVE, RhoGEF and PH domain containing 2
Calculated molecular weight 655 aa, 75 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC023645
Gene symbol FGD2
Gene ID (NCBI) 221472
RRIDAB_2880740
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FGD2 is a member of the formin family of proteins, which are known for their role in regulating the assembly of actin filaments. Formins are characterized by their ability to nucleate and elongate actin filaments, and they play a crucial role in processes that require dynamic actin structures, such as cell motility and cell division. Mutations or alterations in FGD2 have been associated with certain developmental disorders and diseases. For example, mutations in FGD2 are a cause of faciogenital dysplasia (Aarskog-Scott syndrome), a condition characterized by facial and genital abnormalities and short stature.

Protocols

Product Specific Protocols
WB protocol for FGD2 antibody 27068-1-APDownload protocol
IHC protocol for FGD2 antibody 27068-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols