Validation Data Gallery
Tested Applications
| Positive IHC detected in | human lung tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
20910-1-AP targets FGD5 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FGD5 fusion protein Ag15037 相同性解析による交差性が予測される生物種 |
| Full Name | FYVE, RhoGEF and PH domain containing 5 |
| Calculated molecular weight | 1462 aa, 160 kDa |
| GenBank accession number | BC035364 |
| Gene Symbol | FGD5 |
| Gene ID (NCBI) | 152273 |
| RRID | AB_10733757 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6ZNL6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FGD5 (or ZFYVE23) is an endothelial specific gene, plays a crucial role in vascular pruning. Deficient expression of FGD5 markedly inhibits neovascularization, indicated by in vitro tube-formation, aortic-ring, and coated-bead assays and by in vivo coated-bead plug assays and studies in the murine retina model. FGD5-deficient endothelial cells fail to migrate. Moreover, the cells are susceptible to proapoptotic stimuli associated with VEGF-stimulated activation of Akt. FGD5 promotes apoptosis-induced vaso-obliteration via induction of the hey1-p53 pathway by direct binding and activation of CDC42.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for FGD5 antibody 20910-1-AP | Download protocol |
| IF protocol for FGD5 antibody 20910-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO Rep VE-PTP inhibition stabilizes endothelial junctions by activating FGD5.
| ||
Arterioscler Thromb Vasc Biol Facio-genital dysplasia-5 regulates matrix adhesion and survival of human endothelial cells. | ||
Arterioscler Thromb Vasc Biol FGD5 Regulates VEGF Receptor-2 Coupling to PI3 Kinase and Receptor Recycling.
| ||
Dev Cell FGD5 in basal cells induces CXCL14 secretion that initiates a feedback loop to promote murine mammary epithelial growth and differentiation |