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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FGD6 Recombinant antibody

FGD6 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240260A6

Cat no : 83296-4-RR

Synonyms

Zinc finger FYVE domain-containing protein 24, ZFYVE24, FYVE, RhoGEF and PH domain-containing protein 6, 240260A6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FGD6 Recombinant antibody (83296-4-RR)

    WB analysis using 83296-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83296-4-RR (FGD6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83296-4-RR

    Affinity and Kinetic Characterization of 83296-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83296-4-RR against Human FGD6 were performed. The affinity constant is 10.7 nM.

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Tested Applications

Positive WB detected inK-562 cells, A549 cells, Jurkat cells, HeLa cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83296-4-RR is FGD6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FGD6 fusion protein Ag22211 相同性解析による交差性が予測される生物種
Full Name FYVE, RhoGEF and PH domain containing 6
Calculated molecular weight 1430 aa, 161 kDa
Observed molecular weight 161 kDa
GenBank accession numberBC013319
Gene symbol FGD6
Gene ID (NCBI) 55785
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FGD6, also known as ZFYVE24, is localized on the Homo sapiens chromosome 12q22 and consists of 1,400 amino acids. Previous studies have reported that variations in FGD6 increase individual susceptibility to polypoidal choroidal vasculopathy. It was also demonstrated that FGD6 coordinates cell polarity and endosomal membrane recycling in osteoclasts by regulating the assembly of different actin-based protein networks and activating Cdc42 at different locations.

Protocols

Product Specific Protocols
WB protocol for FGD6 antibody 83296-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols