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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FGF-2 Polyclonal antibody

FGF-2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30333-1-AP

Synonyms

Basic fibroblast growth factor, BFGF, FGF2, FGF-2, FGFB, HBGF 2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using FGF-2 Polyclonal antibody (30333-1-AP)

    WB analysis using 30333-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30333-1-AP (FGF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of SKOV-3 cells using FGF-2 Polyclonal antibody (30333-1-AP)

    IF Staining of SKOV-3 using 30333-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using FGF-2 antibody (30333-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inK-562 cells, SKOV-3 cells, U-87 MG cells
Positive IF/ICC detected inSKOV-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

30333-1-AP targets FGF-2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FGF-2 fusion protein Ag28653 相同性解析による交差性が予測される生物種
Full Name fibroblast growth factor 2 (basic)
Calculated molecular weight 31 aa
Observed molecular weight21 kDa，23 kDa
GenBank accession numberNM_002006
Gene symbol FGF2
Gene ID (NCBI) 2247
RRIDAB_3669711
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fibroblast growth factor-2 (FGF-2), also referred to as basic FGF, belongs to a family of growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in at least five different isoforms with distinct properties.

Protocols

Product Specific Protocols
WB protocol for FGF-2 antibody 30333-1-APDownload protocol
IF protocol for FGF-2 antibody 30333-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Bioengineering (Basel)

Shark Cartilage-Derived Anti-Angiogenic Peptide Inhibits Corneal Neovascularization

Authors - Yunxian Li
View Article