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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FGF10 Polyclonal antibody

FGF10 Polyclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 29749-1-AP

Synonyms

FGF 10, FGF10, fibroblast growth factor 10, Keratinocyte growth factor 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FGF10 Polyclonal antibody (29749-1-AP)

    WB analysis using 29749-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29749-1-AP (FGF10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using FGF10 Polyclonal antibody (29749-1-AP)

    IF Staining of mouse brain using 29749-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using FGF10 antibody (29749-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, NIH/3T3 cells
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29749-1-AP targets FGF10 in WB, IF-P, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FGF10 fusion protein Ag31278 相同性解析による交差性が予測される生物種
Full Name fibroblast growth factor 10
Observed molecular weight40 kDa
GenBank accession numberBC105021
Gene symbol FGF10
Gene ID (NCBI) 2255
RRIDAB_2918343
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FGF10 belongs to the FGF7 subfamily. FGF10 is a paracrine signaling growth factor of 215 amino acids with a typical signal sequence for secretion and plays an essential role during development and tissue homeostasis in adults (PMID: 30405705). FGF10 is mainly produced by neuron and microglia/macrophages (PMID: 28981091). The calculated molecular weight of FGF10 is 23 kDa. Sometimes higher molecular weight around 40 kDa can also be observed, which may be a modified variant of FGF10.

Protocols

Product Specific Protocols
WB protocol for FGF10 antibody 29749-1-APDownload protocol
IF protocol for FGF10 antibody 29749-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols