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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FGF12 Monoclonal antibody, PBS Only

FGF12 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F11A10

Cat no : 60152-1-PBS

Synonyms

FGF 12, FGF12, FGF12B, FHF 1, FHF1, fibroblast growth factor 12, Myocyte activating factor

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using FGF12 Monoclonal antibody (60152-1-Ig)

    WB analysis of mouse brain using 60152-1-Ig (same clone as 60152-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 60152-1-Ig (FGF12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60152-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using FGF12 Monoclonal antibody (60152-1-Ig)

    WB analysis of human brain using 60152-1-Ig (same clone as 60152-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 60152-1-Ig (FGF12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60152-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat brain tissue using FGF12 Monoclonal antibody (60152-1-Ig)

    WB analysis of rat brain using 60152-1-Ig (same clone as 60152-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 60152-1-Ig (FGF12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60152-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using FGF12 Monoclonal antibody (60152-1-Ig)

    FC experiment of HeLa using 60152-1-Ig (same clone as 60152-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug FGF12 Monoclonal antibody (60152-1-Ig, Clone:2F11A10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60152-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60152-1-PBS targets FGF12 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen FGF12 fusion protein Ag5046 相同性解析による交差性が予測される生物種
Full Name fibroblast growth factor 12
Calculated molecular weight 181 aa, 20 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC022524
Gene symbol FGF12
Gene ID (NCBI) 2257
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

FGF12 is a member of the fibroblast growth factor (FGF) family. FGF family members play important roles in embryogenesis, angiogenesis, and wound repair. FGF12 lacks the N-terminal signal sequence present in most of the FGF family members, but it contains clusters of basic residues that have been demonstrated to act as a nuclear localization signal. When transfected into mammalian cells, this protein accumulated in the nucleus, but was not secreted. FGF12 plays an intracellular role in the inhibition of radiation-induced apoptosis. FGF12 is expressed abundantly in developing and adult nervous systems; therefore, FGF12 was thought to be related to nervous system development and function.