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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using FGF17 Polyclonal antibody (25314-1-AP)

    WB analysis of MCF-7 using 25314-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25314-1-AP (FGF17 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using FGF17 Polyclonal antibody (25314-1-AP)

    WB analysis of PC-3 using 25314-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 25314-1-AP (FGF17 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using FGF17 Polyclonal antibody (25314-1-AP)

    IF Staining of NIH/3T3 using 25314-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using FGF17 antibody (25314-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

FGF17 Polyclonal antibody

FGF17 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25314-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

FGF 17, FGF-17, Fibroblast growth factor 17, UNQ161/PRO187

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, PC-3 cells
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

25314-1-AP targets FGF17 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FGF17 fusion protein Ag17807 相同性解析による交差性が予測される生物種
Full Name fibroblast growth factor 17
Calculated molecular weight 216 aa, 25 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC105131
Gene Symbol FGF17
Gene ID (NCBI) 8822
RRIDAB_2880025
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60258
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FGF17 antibody 25314-1-APDownload protocol
IF protocol for FGF17 antibody 25314-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Cell

Ex utero monkey embryogenesis from blastocyst to early organogenesis

Authors - Yandong Gong
View Article