Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | mouse liver tissue, L02 cells, HeLa cells, rat liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
20711-1-AP targets FGF-8 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | fibroblast growth factor 8 (androgen-induced) |
| Calculated molecular weight | 27 kDa |
| Observed molecular weight | 24 kDa |
| GenBank accession number | NM_006119 |
| Gene Symbol | FGF8 |
| Gene ID (NCBI) | 2253 |
| RRID | AB_10733225 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P55075 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FGF8, also named as AIGF and HBGF-8, belongs to the heparin-binding growth factors family. FGF8 stimulates growth of the cells in an autocrine manner. It mediates hormonal action on the growth of cancer cells. Defects in FGF8 are the cause of Kallmann syndrome type 6 (KAL6). Defects in FGF8 are a cause of idiopathic hypogonadotropic hypogonadism (IHH). The antibody is specific to FGF8.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FGF-8 antibody 20711-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Carcinogenesis The oncoprotein HBXIP enhances angiogenesis and growth of breast cancer through modulating FGF8 and VEGF. | ||
Oncoimmunology Immunoreactivity against fibroblast growth factor 8 in alveolar rhabdomyosarcoma patients and its involvement in tumor aggressiveness. | ||
Oncol Lett Systematic analysis of expression profiles and prognostic significance of the FGF gene family in pancreatic adenocarcinoma |