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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FGFR1 Polyclonal antibody

FGFR1 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 30358-1-AP

Synonyms

EC:2.7.10.1, CEK, CD331, bFGF-R-1, BFGFR

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of SH-SY5Y cells using FGFR1 Polyclonal antibody (30358-1-AP)

    IP experiment of SH-SY5Y using 30358-1-AP

    IP result of anti-FGFR1 (IP:30358-1-AP, 4ug; Detection:30358-1-AP 1:300) with SH-SY5Y cells lysate 770 ug.

  • Immunohistochemistry (IHC) staining of human colon tissue using FGFR1 Polyclonal antibody (30358-1-AP)

    IHC staining of human colon using 30358-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 30358-1-AP (FGFR1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using FGFR1 Polyclonal antibody (30358-1-AP)

    IHC staining of human colon using 30358-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 30358-1-AP (FGFR1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using FGFR1 Polyclonal antibody (30358-1-AP)

    IF Staining of SH-SY5Y using 30358-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using FGFR1 antibody (30358-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive IP detected inSH-SY5Y cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30358-1-AP targets FGFR1 in IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name fibroblast growth factor receptor 1
Calculated molecular weight 820 aa, 91 kDa
Observed molecular weight80 kDa, 95 kDa, 105 kDa
GenBank accession numberBC018128
Gene symbol FGFR1
Gene ID (NCBI) 2260
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fibroblast growth factor receptor 1 (FGFR1) is a tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors. FGFR1 has been implicated in diverse biological processes including embryonic development, cell proliferation, differentiation, migration, and tumorigenesis. Mutations in the FGFR1 gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, squamous cell lung cancer, and autosomal dominant Kallmann syndrome. Alternatively spliced variants that encode different protein isoforms have been described.

Protocols

Product Specific Protocols
IHC protocol for FGFR1 antibody 30358-1-APDownload protocol
IF protocol for FGFR1 antibody 30358-1-APDownload protocol
IP protocol for FGFR1 antibody 30358-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols