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KD/KO Validated

FGFR1OP Polyclonal antibody

Name: FGFR1OP Polyclonal antibody
Brand: Proteintech
SKU: 11343-1-AP

FGFR1OP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11343-1-AP

Synonyms

FOP, FGFR1 oncogene partner, CEP43, Centrosomal protein 43

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 11343-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11343-1-AP (FGFR1OP antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11343-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11343-1-AP (FGFR1OP Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of U-937 using 11343-1-AP
U-937 cells were subjected to SDS PAGE followed by western blot with 11343-1-AP (FGFR1OP Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 11343-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 11343-1-AP (FGFR1OP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC staining of human lymphoma using 11343-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma using 11343-1-AP (FGFR1OP antibody) at dilution of 1:50 (under 10x lens).
IF Staining of HeLa using 11343-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FGFR1OP antibody (11343-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HEK-293 using 11343-1-AP
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using FGFR1OP antibody (11343-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 11343-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, mouse heart tissue, U-937 cells, HL-60 cells, MCF-7 cells, HeLa cells
Positive IHC detected in	human lymphoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 6 publications below
IHC	See 1 publications below
IF	See 10 publications below

Product Information

11343-1-AP targets FGFR1OP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	FGFR1OP fusion protein Ag1891 相同性解析による交差性が予測される生物種
Full Name	FGFR1 oncogene partner
Calculated molecular weight	43 kDa
Observed molecular weight	50 kDa
GenBank accession number	BC011902
Gene symbol	FGFR1OP
Gene ID (NCBI)	11116
RRID	AB_2103362
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The fibroblast growth factor receptor 1 oncogene partner (FGFR1OP), FOP, is a centrosomal protein that is involved in the anchoring of microtubules to subcellular structures. Three isoforms have been produced by alternative splicing. A chromosomal aberration involving FGFR1OP may be a cause of stem cell myeloproliferative disorder (MPD). MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma and progresses to acute myeloid leukemia.

Protocols

Product Specific Protocols
WB protocol for FGFR1OP antibody 11343-1-AP	Download protocol
IHC protocol for FGFR1OP antibody 11343-1-AP	Download protocol
IF protocol for FGFR1OP antibody 11343-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
zebrafish	IF	Cell Vertebrate cells differentially interpret ciliary and extraciliary cAMP. Authors - Melissa E Truong View Article
human	WB,IF	Nat Commun Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis. Authors - Hai-Qing Tu KD Validated View Article
mouse	IF	Dev Cell Endoderm development requires centrioles to restrain p53-mediated apoptosis in the absence of ERK activity. Authors - Chang Xie View Article
human	WB	Dev Cell The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base. Authors - Tomoharu Kanie View Article
human	IF	J Cell Biol A ciliopathy complex builds distal appendages to initiate ciliogenesis. Authors - Dhivya Kumar View Article
human,mouse	IF	Elife Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis Authors - Emma A Hall View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FGFR1OP Polyclonal antibody

FGFR1OP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 11343-1-AP

WB analysis of HEK-293 using 11343-1-AP

WB analysis of U-937 using 11343-1-AP

WB analysis of mouse heart using 11343-1-AP

IHC staining of human lymphoma using 11343-1-AP

IF Staining of HeLa using 11343-1-AP

IF Staining of HEK-293 using 11343-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Vertebrate cells differentially interpret ciliary and extraciliary cAMP.

Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.

Endoderm development requires centrioles to restrain p53-mediated apoptosis in the absence of ERK activity.

The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base.

A ciliopathy complex builds distal appendages to initiate ciliogenesis.

Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis