Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, mouse heart tissue, U-937 cells, HL-60 cells, MCF-7 cells, HeLa cells |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
| IF | See 13 publications below |
Product Information
11343-1-AP targets FGFR1OP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FGFR1OP fusion protein Ag1891 相同性解析による交差性が予測される生物種 |
| Full Name | FGFR1 oncogene partner |
| Calculated molecular weight | 43 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC011902 |
| Gene Symbol | FGFR1OP |
| Gene ID (NCBI) | 11116 |
| RRID | AB_2103362 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95684 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The fibroblast growth factor receptor 1 oncogene partner (FGFR1OP), FOP, is a centrosomal protein that is involved in the anchoring of microtubules to subcellular structures. Three isoforms have been produced by alternative splicing. A chromosomal aberration involving FGFR1OP may be a cause of stem cell myeloproliferative disorder (MPD). MPD is characterized by myeloid hyperplasia, eosinophilia and T-cell or B-cell lymphoblastic lymphoma and progresses to acute myeloid leukemia.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FGFR1OP antibody 11343-1-AP | Download protocol |
| IHC protocol for FGFR1OP antibody 11343-1-AP | Download protocol |
| IF protocol for FGFR1OP antibody 11343-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.
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Dev Cell Endoderm development requires centrioles to restrain p53-mediated apoptosis in the absence of ERK activity. | ||
Dev Cell The CEP19-RABL2 GTPase Complex Binds IFT-B to Initiate Intraflagellar Transport at the Ciliary Base. | ||