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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FGFR2 Recombinant antibody (84205-4-RR)

    WB analysis using 84205-4-RR (same clone as 84205-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84205-4-RR (FGFR2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84205-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using FGFR2 Recombinant antibody (84205-4-RR)

    IHC staining of mouse liver using 84205-4-RR (same clone as 84205-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84205-4-RR (FGFR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84205-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01128-1

    Cytometric bead array standard curve of MP01128-1

    Cytometric bead array standard curve of MP01128-1, FGFR2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84205-3-PBS. Detection antibody: 84205-4-PBS. Standard: Eg1265. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP01128-3

    Cytometric bead array standard curve of MP01128-3

    Cytometric bead array standard curve of MP01128-3, FGFR2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84205-2-PBS. Detection antibody: 84205-4-PBS. Standard: Eg1265. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 84205-4-RR

    Affinity and Kinetic Characterization of 84205-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84205-4-RR against Human FGFR2 were performed. The affinity constant is 18.8 nM.

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FGFR2 Recombinant antibody, PBS Only (Detector)

FGFR2 Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 84205-4-PBS
Clone No.241383G9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Synonyms

CFD1, CEK3, CD332, BFR 1, BEK

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84205-4-PBS targets FGFR2 as part of a matched antibody pair:

MP01128-1: 84205-3-PBS capture and 84205-4-PBS detection (validated in Cytometric bead array)

MP01128-3: 84205-2-PBS capture and 84205-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name fibroblast growth factor receptor 2
Calculated molecular weight92kd
Observed molecular weight85 kDa
GenBank accession numberNM_000141.4
Gene Symbol FGFR2
Gene ID (NCBI) 2263
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP21802
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

FGFR2 (Fibroblast growth factor receptor 2) is a tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration, and apoptosis. Ligand binding leads to the activation of several signaling pathways, such as RAS, MAPK1/ERK2, MAPK3/ERK1, and the MAP Kinase signaling pathway, as well as the AKT1 signaling pathway. Mutations in the gene of FGFR2 are associated with Crouzon syndrome, Pfeiffer syndrome, Craniosynostosis, Apert syndrome, Jackson-Weiss syndrome, Beare-Stevenson cutis gyrata syndrome, Saethre-Chotzen syndrome, and syndromic craniosynostosis.