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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FGFR4 Recombinant antibody

FGFR4 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

5P12

Cat no : 81069-1-RR

Synonyms

JTK2, FGFR-4, FGFR 4, EC:2.7.10.1, CD334

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FGFR4 Recombinant antibody (81069-1-RR)

    WB analysis using 81069-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81069-1-RR (FGFR4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 81069-1-RR

    Affinity and Kinetic Characterization of 81069-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81069-1-RR against Human FGFR4 were performed. The affinity constant is 25 nM.

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Tested Applications

Positive WB detected inMOLT-4 cells, Raji cells, Jurkat cells, HeLa cells, HepG2 cells, HT-29 cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81069-1-RR targets FGFR4 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FGFR4 fusion protein Ag31013 相同性解析による交差性が予測される生物種
Full Name fibroblast growth factor receptor 4
Calculated molecular weight 88 kDa
Observed molecular weight100-110 kDa
GenBank accession numberBC011847
Gene symbol FGFR4
Gene ID (NCBI) 2264
RRIDAB_2918926
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FGFR4 antibody 81069-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols