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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FGL1 Monoclonal antibody

FGL1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B11C6

Cat no : 67391-1-Ig

Synonyms

FGL1, fibrinogen like 1, Fibrinogen like protein 1, Hepassocin, HFREP 1, HFREP1, HP 041, LFIRE 1, LFIRE1

Validation Data Gallery

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  • Western Blot (WB) analysis of pig liver tissue using FGL1 Monoclonal antibody (67391-1-Ig)

    WB analysis of pig liver using 67391-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67391-1-Ig (FGL1 antibody) at dilution of 1:2700 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using FGL1 Monoclonal antibody (67391-1-Ig)

    WB analysis of human placenta using 67391-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67391-1-Ig (FGL1 antibody) at dilution of 1:2700 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using FGL1 Monoclonal antibody (67391-1-Ig)

    IHC staining of human liver using 67391-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67391-1-Ig (FGL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using FGL1 Monoclonal antibody (67391-1-Ig)

    IHC staining of human liver using 67391-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67391-1-Ig (FGL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using FGL1 Monoclonal antibody (67391-1-Ig)

    IHC staining of human lung using 67391-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 67391-1-Ig (FGL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung tissue using FGL1 Monoclonal antibody (67391-1-Ig)

    IHC staining of human lung using 67391-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 67391-1-Ig (FGL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FGL1 Monoclonal antibody (67391-1-Ig)

    IHC staining of human liver cancer using 67391-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67391-1-Ig (FGL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using FGL1 Monoclonal antibody (67391-1-Ig)

    IHC staining of human liver cancer using 67391-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67391-1-Ig (FGL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FGL1 Monoclonal antibody (67391-1-Ig)

    IF Staining of HepG2 using 67391-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FGL1 antibody (67391-1-Ig, Clone: 2B11C6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inpig liver tissue, human placenta tissue
Positive IHC detected inhuman liver tissue, human lung tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5400
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67391-1-Ig targets FGL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, pig samples.

Tested Reactivity Human, pig
Cited Reactivity mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FGL1 fusion protein Ag8581 相同性解析による交差性が予測される生物種
Full Name fibrinogen-like 1
Calculated molecular weight 312 aa, 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC007047
Gene symbol FGL1
Gene ID (NCBI) 2267
RRIDAB_2882635
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

Fibrinogen-like 1 (FGL1), also termed hepatocyte-derived fibrinogen-related protein 1 (HFREP1) or hepassocin, is a predominantly liver expressed protein that has been implicated as both a hepatoprotectant and a hepatocyte mitogen. FGL1 expression is decreased in hepatocellular carcinoma (HCC), and it may play a role in the development of hepatocellular carcinomas.

Protocols

Product Specific Protocols
WB protocol for FGL1 antibody 67391-1-IgDownload protocol
IHC protocol for FGL1 antibody 67391-1-IgDownload protocol
IF protocol for FGL1 antibody 67391-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Ther Oncolytics

Dual-targeting vaccine of FGL1/CAIX exhibits potent anti-tumor activity by activating DC-mediated multi-functional CD8 T cell immunity

Authors - Dafei Chai
View Article