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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FGR Polyclonal antibody (31641-1-AP)

    WB analysis using 31641-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31641-1-AP (FGR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using FGR Polyclonal antibody (31641-1-AP)

    IF Staining of Raji using 31641-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using FGR antibody (31641-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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FGR Polyclonal antibody

FGR Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31641-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Proto oncogene c Fgr, p58c fgr, p55c fgr, p55 Fgr, c src2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHL-60 cells, Raji cells
Positive IF/ICC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31641-1-AP targets FGR in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FGR fusion protein Ag34944 相同性解析による交差性が予測される生物種
Full Name Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog
Calculated molecular weight59 kDa
Observed molecular weight59 kDa
GenBank accession numberNM_005248
Gene Symbol FGR
Gene ID (NCBI) 2268
RRIDAB_3670061
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP09769
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FGR (Tyrosine-protein kinase Fgr), a member of the Src family of tyrosine kinases, is highly expressed in many cancers and can regulate mitochondrial oxidative phosphorylation (PMID: 37475042).

Protocols

Product Specific Protocols
WB protocol for FGR antibody 31641-1-APDownload protocol
IF protocol for FGR antibody 31641-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols