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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FGR Recombinant antibody

FGR Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240275E5

Cat no : 83352-1-RR

Synonyms

p58c fgr, p55c fgr, p55 Fgr, c src2, c fgr

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FGR Recombinant antibody (83352-1-RR)

    WB analysis using 83352-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83352-1-RR (FGR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using FGR Recombinant antibody (83352-1-RR)

    IF Staining of Raji using 83352-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using FGR antibody (83352-1-RR, Clone: 240275E5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of Raji cells using FGR Recombinant antibody (83352-1-RR)

    FC experiment of Raji using 83352-1-RR

    1x10^6 Raji cells were intracellularly stained with 0.25 ug FGR Recombinant antibody (83352-1-RR, Clone:240275E5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83352-1-RR

    Affinity and Kinetic Characterization of 83352-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83352-1-RR against Human FGR were performed. The affinity constant is 0.139 nM.

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Tested Applications

Positive WB detected inDaudi cells, Raji cells
Positive IF/ICC detected inRaji cells
Positive FC (Intra) detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83352-1-RR is FGR Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FGR fusion protein Ag34944 相同性解析による交差性が予測される生物種
Full Name Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog
Calculated molecular weight59 kDa
Observed molecular weight60 kDa
GenBank accession numberNM_005248
Gene symbol FGR
Gene ID (NCBI) 2268
RRIDAB_3671010
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FGR (Tyrosine-protein kinase Fgr) is a member of the Src family of protein tyrosine kinases (PTKs). It's contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively.

Protocols

Product Specific Protocols
WB protocol for FGR antibody 83352-1-RRDownload protocol
IF protocol for FGR antibody 83352-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols