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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FH Polyclonal antibody

FH Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10966-1-AP

Synonyms

HsFH, Fumarase, EC:4.2.1.2

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using FH Polyclonal antibody (10966-1-AP)

    WB analysis of HeLa using 10966-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using FH Polyclonal antibody (10966-1-AP)

    WB analysis of L02 using 10966-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using FH Polyclonal antibody (10966-1-AP)

    WB analysis of mouse liver using 10966-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using FH Polyclonal antibody (10966-1-AP)

    WB analysis of A431 using 10966-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using FH Polyclonal antibody (10966-1-AP)

    WB analysis of HepG2 using 10966-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using FH Polyclonal antibody (10966-1-AP)

    WB analysis of human liver using 10966-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using FH Polyclonal antibody (10966-1-AP)

    WB analysis of HEK-293 using 10966-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10966-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using FH Polyclonal antibody (10966-1-AP)

    IP experiment of HepG2 using 10966-1-AP

    IP result of anti-FH (IP:10966-1-AP, 4ug; Detection:10966-1-AP 1:1000) with HepG2 cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using FH Polyclonal antibody (10966-1-AP)

    IHC staining of human prostate cancer using 10966-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10966-1-AP (FH antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using FH Polyclonal antibody (10966-1-AP)

    IHC staining of human liver using 10966-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10966-1-AP (FH Antibody) at dilution of 1:50(under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using FH Polyclonal antibody (10966-1-AP)

    IHC staining of human liver using 10966-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10966-1-AP (FH Antibody) at dilution of 1:50(under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FH Polyclonal antibody (10966-1-AP)

    IF Staining of HepG2 using 10966-1-AP

    Immunofluorescent analysis of HepG2 cells, using FH antibody 10966-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FH Polyclonal antibody (10966-1-AP)

    IF Staining of HepG2 using 10966-1-AP

    Immunofluorescent analysis of HepG2 cells using 10966-1-AP (FH antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, A431 cells, HEK-293 cells, HepG2 cells, human liver tissue, L02 cells, mouse liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman prostate cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10966-1-AP targets FH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FH fusion protein Ag1437 相同性解析による交差性が予測される生物種
Full Name fumarate hydratase
Calculated molecular weight 55 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC017444
Gene symbol FH
Gene ID (NCBI) 2271
RRIDAB_568948
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FH(fumarate hydratase) belongs to the class-II fumarase/aspartase family. It is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. FH also acts a tumor suppressor functions of FH in sporadic kidney cancer(PMID:21695080). This protein has 2 isoforms produced by alternative initiation.

Protocols

Product Specific Protocols
WB protocol for FH antibody 10966-1-APDownload protocol
IHC protocol for FH antibody 10966-1-APDownload protocol
IF protocol for FH antibody 10966-1-APDownload protocol
IP protocol for FH antibody 10966-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

Identification of SPOP related metabolic pathways in prostate cancer.

Authors - Min Yan
View Article
humanIF

Endocr Relat Cancer

Cellular kinetics of MED12-mutant uterine leiomyoma growth and regression in vivo.

Authors - Vanida Ann Serna
View Article
human,mouseWB,IHC

Pharmaceuticals (Basel)

Identification of a Difluorinated Alkoxy Sulfonyl Chloride as a Novel Antitumor Agent for Hepatocellular Carcinoma through Activating Fumarate Hydratase Activity

Authors - Jin Jin
View Article