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KD/KO Validated

FH Polyclonal antibody

Name: FH Polyclonal antibody
Brand: Proteintech
SKU: 11375-1-AP

FH Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11375-1-AP

Synonyms

HsFH, Fumarase, EC:4.2.1.2

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 11375-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11375-1-AP (FH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11375-1-AP
WB result of FH antibody (11375-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FH transfected HepG2 cells.
WB analysis using 11375-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11375-1-AP (FH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 11375-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 11375-1-AP (FH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 11375-1-AP
IP result of anti-FH (IP:11375-1-AP, 4ug; Detection:11375-1-AP 1:8000) with mouse liver tissue lysate 3040 ug.
IHC staining of human placenta using 11375-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11375-1-AP (FH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 11375-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11375-1-AP (FH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 11375-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11375-1-AP (FH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 11375-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11375-1-AP (FH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human normal colon using 11375-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11375-1-AP (FH antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 11375-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FH antibody (11375-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Cat No. 11375-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, HepG2 cells, human placenta tissue, Hela cells, Jurkat cells, NIH/3T3 cells, C6 cells, mouse liver tissue, rat liver tissue, L02 cells
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human placenta tissue, human normal colon, human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 11 publications below
IHC	See 4 publications below
IF	See 1 publications below

Product Information

11375-1-AP targets FH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, caenorhabditis elegans
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	FH fusion protein Ag1934 相同性解析による交差性が予測される生物種
Full Name	fumarate hydratase
Calculated molecular weight	510 aa, 55 kDa
Observed molecular weight	45-50 kDa
GenBank accession number	BC003108
Gene symbol	FH
Gene ID (NCBI)	2271
RRID	AB_763171
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Fumarate hydratase, or fumarase, is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. It belongs to the class-II fumarase/aspartase family. This protein has 2 isoforms produced by alternative initiation.

Protocols

Product Specific Protocols
WB protocol for FH antibody 11375-1-AP	Download protocol
IHC protocol for FH antibody 11375-1-AP	Download protocol
IF protocol for FH antibody 11375-1-AP	Download protocol
IP protocol for FH antibody 11375-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1. Authors - Xiaoyu Ma View Article
mouse	WB	Aging Dis Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging Authors - Minghao Yuan View Article
human	WB	Theranostics EBV(LMP1)-induced metabolic reprogramming inhibits necroptosis through the hypermethylation of the RIP3 promoter. Authors - Feng Shi View Article
human	WB	Oncotarget CDC5L drives FAH expression to promote metabolic reprogramming in melanoma. Authors - Zhichao Gu View Article
human	WB	Invest Ophthalmol Vis Sci Proteomics of uveal melanomas suggests HSP-27 as a possible surrogate marker of chromosome 3 loss. Authors - Coupland Sarah E SE View Article
Caenorhabditis elegans	WB	Int J Radiat Biol Coupling of oxidative stress responses to tricarboxylic acid cycle and prostaglandin E2 alterations in Caenorhabditis elegans under extremely low-frequency electromagnetic field. Authors - Yongyan Sun View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FH Polyclonal antibody

FH Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 11375-1-AP

WB analysis of HepG2 using 11375-1-AP

WB analysis using 11375-1-AP

WB analysis of human placenta using 11375-1-AP

IP experiment of mouse liver using 11375-1-AP

IHC staining of human placenta using 11375-1-AP

IHC staining of human placenta using 11375-1-AP

IHC staining of human ovary tumor using 11375-1-AP

IHC staining of human ovary tumor using 11375-1-AP

IHC staining of human normal colon using 11375-1-AP

IF Staining of HeLa using 11375-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Lin28/let-7 axis regulates aerobic glycolysis and cancer progression via PDK1.

Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging

EBV(LMP1)-induced metabolic reprogramming inhibits necroptosis through the hypermethylation of the RIP3 promoter.

CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.

Proteomics of uveal melanomas suggests HSP-27 as a possible surrogate marker of chromosome 3 loss.

Coupling of oxidative stress responses to tricarboxylic acid cycle and prostaglandin E2 alterations in Caenorhabditis elegans under extremely low-frequency electromagnetic field.