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  • Featured Product
  • KD/KO Validated

FH Monoclonal antibody

FH Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A9C8

Cat no : 68162-1-Ig

Synonyms

HsFH, Fumarase, EC:4.2.1.2, 1A9C8

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FH Monoclonal antibody (68162-1-Ig)

    WB analysis using 68162-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68162-1-Ig (FH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of HepG2 cells using FH Monoclonal antibody (68162-1-Ig)

    WB analysis of HepG2 using 68162-1-Ig

    WB result of FH antibody (68162-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FH transfected HepG2 cells.

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Tested Applications

Positive WB detected inA431 cells, HepG2 cells, LNCaP cells, HEK-293 cells, Jurkat cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68162-1-Ig targets FH in WB, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen FH fusion protein Ag30254 相同性解析による交差性が予測される生物種
Full Name fumarate hydratase
Calculated molecular weight 510 aa, 55 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC003108
Gene symbol FH
Gene ID (NCBI) 2271
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fumarate hydratase, or fumarase, is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. It belongs to the class-II fumarase/aspartase family. This protein has 2 isoforms produced by alternative initiation.

Protocols

Product Specific Protocols
WB protocol for FH antibody 68162-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols