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Species Reactivity

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  • Featured Product
  • KD/KO Validated

FH Recombinant antibody

FH Recombinant Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Rat, Mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

CloneNo.

3K16

Cat no : 82508-1-RR

Synonyms

HsFH, Fumarase, EC:4.2.1.2, 3K16

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using FH Recombinant antibody (82508-1-RR)

    WB analysis of HepG2 using 82508-1-RR

    WB result of FH antibody (82508-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FH transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using FH Recombinant antibody (82508-1-RR)

    WB analysis using 82508-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82508-1-RR (FH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using FH Recombinant antibody (82508-1-RR)

    IP experiment of mouse liver using 82508-1-RR

    IP result of anti-FH (IP:82508-1-RR, 4ug; Detection:82508-1-RR 1:4000) with mouse liver tissue lysate 1840 ug.

  • Immunohistochemistry (IHC) staining of human stomach tissue using FH Recombinant antibody (82508-1-RR)

    IHC staining of human stomach using 82508-1-RR

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82508-1-RR (FH antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82508-1-RR

    Affinity and Kinetic Characterization of 82508-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82508-1-RR against Human FH were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, A431 cells, L02 cells, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82508-1-RR targets FH in WB, IP, ELISA applications and shows reactivity with Human, Rat, Mouse samples.

Tested Reactivity Human, Rat, Mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FH fusion protein Ag1934 相同性解析による交差性が予測される生物種
Full Name fumarate hydratase
Calculated molecular weight 510 aa, 55 kDa
Observed molecular weight45 kDa
GenBank accession numberBC003108
Gene symbol FH
Gene ID (NCBI) 2271
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FH(fumarate hydratase) belongs to the class-II fumarase/aspartase family. It is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. FH also acts as a tumor suppressor function of FH in sporadic kidney cancer(PMID:21695080). Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.

Protocols

Product Specific Protocols
WB protocol for FH antibody 82508-1-RRDownload protocol
IP protocol for FH antibody 82508-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols