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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FHIT Recombinant antibody (83710-1-RR)

    WB analysis using 83710-1-RR (same clone as 83710-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83710-1-RR (FHIT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83710-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FHIT Recombinant antibody (83710-1-RR)

    IHC staining of human colon cancer using 83710-1-RR (same clone as 83710-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83710-1-RR (FHIT antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83710-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83710-1-RR

    Affinity and Kinetic Characterization of 83710-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83710-1-RR against Human FHIT were performed. The affinity constant is 5.01 nM.

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FHIT Recombinant antibody, PBS Only

FHIT Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 83710-1-PBS
Clone No.240564D9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

AP3Aase, AP3A hydrolase, Adenylylsulfate-ammonia adenylyltransferase, Adenylylsulfatase, Adenosine 5'-monophosphoramidase FHIT

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83710-1-PBS targets FHIT in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FHIT fusion protein Ag4181 相同性解析による交差性が予測される生物種
Full Name fragile histidine triad gene
Calculated molecular weight 147 aa, 17 kDa
Observed molecular weight15-17 kDa
GenBank accession numberBC032336
Gene Symbol FHIT
Gene ID (NCBI) 2272
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP49789
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The candidate tumor suppressor gene, FHIT(Fragile histidine triad protein), encompasses the common human chromosomal fragile site at 3p14.2, the hereditary renal cancer translocation breakpoint, and cancer cell homozygous deletions. Fhit hydrolyzes dinucleotide 5',5'''-P1,P3-triphosphate in vitro and mutation of a central histidine abolishes hydrolase activity(PMID:9391102). FHIT has a major role in regulating beta-catenin-mediated gene transcription.