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KD/KO Validated

FHL2 Polyclonal antibody

Name: FHL2 Polyclonal antibody
Brand: Proteintech
SKU: 21619-1-AP

FHL2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 21619-1-AP

Synonyms

Skeletal muscle LIM protein 3, LIM domain protein DRAL, FHL-2, FHL 2, DRAL

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 21619-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21619-1-AP (FHL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IP experiment of mouse skeletal muscle using 21619-1-AP
IP result of anti-FHL2 (IP:21619-1-AP, 4ug; Detection:21619-1-AP 1:1000) with mouse skeletal muscle tissue lysate 2200ug.
IHC staining of rat heart using 21619-1-AP
Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 21619-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 21619-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 21619-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 21619-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 21619-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 21619-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse ovary using 21619-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse ovary using 21619-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat heart using 21619-1-AP
Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat ovary using 21619-1-AP
Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat ovary using 21619-1-AP
Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 21619-1-AP (FHL2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 21619-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 21619-1-AP (FHL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of A431 using 21619-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using FHL2 antibody (21619-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 21619-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, U2OS cells, hTERT-RPE1 cells, NIH/3T3 cells
Positive IP detected in	mouse skeletal muscle tissue
Positive IHC detected in	human cervical cancer tissue, human skin cancer tissue, mouse kidney tissue, mouse ovary tissue, rat heart tissue, rat ovary tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 13 publications below
IHC	See 4 publications below
IF	See 3 publications below
IP	See 1 publications below
CoIP	See 4 publications below

Product Information

21619-1-AP targets FHL2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	FHL2 fusion protein Ag1378 相同性解析による交差性が予測される生物種
Full Name	four and a half LIM domains 2
Calculated molecular weight	32 kDa
Observed molecular weight	32 kDa
GenBank accession number	BC014397
Gene symbol	FHL2
Gene ID (NCBI)	2274
RRID	AB_10860263
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for FHL2 antibody 21619-1-AP	Download protocol
IHC protocol for FHL2 antibody 21619-1-AP	Download protocol
IF protocol for FHL2 antibody 21619-1-AP	Download protocol
IP protocol for FHL2 antibody 21619-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity. Authors - Xiaojie Xu View Article
human	WB,IF,CoIP	EBioMedicine Up-regulated FHL2 inhibits ovulation through interacting with androgen receptor and ERK1/2 in polycystic ovary syndrome. Authors - Ruiqiong Zhou View Article
human,mouse	WB,IHC,IF,CoIP	Cell Death Dis FHL2 deficiency impairs follicular development and fertility by attenuating EGF/EGFR/YAP signaling in ovarian granulosa cells Authors - Chen Wang KD Validated View Article
mouse	CoIP,WB	iScience Genetic ablation of diabetes-associated gene Ccdc92 reduces obesity and insulin resistance in mice Authors - Lu Ren View Article
human	WB	Int J Mol Sci FHL2 Inhibits SARS-CoV-2 Replication by Enhancing IFN-β Expression through Regulating IRF-3 Authors - Zhiqiang Xu KD Validated View Article
human	WB, IHC	J Virol Structural and Functional Characterization of Host FHL1 Protein Interaction with Hypervariable Domain of Chikungunya Virus nsP3 Protein. Authors - Tetyana Lukash View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FHL2 Polyclonal antibody

FHL2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 21619-1-AP

IP experiment of mouse skeletal muscle using 21619-1-AP

IHC staining of rat heart using 21619-1-AP

IHC staining of human cervical cancer using 21619-1-AP

IHC staining of human cervical cancer using 21619-1-AP

IHC staining of human skin cancer using 21619-1-AP

IHC staining of human skin cancer using 21619-1-AP

IHC staining of mouse kidney using 21619-1-AP

IHC staining of mouse kidney using 21619-1-AP

IHC staining of mouse ovary using 21619-1-AP

IHC staining of mouse ovary using 21619-1-AP

IHC staining of rat heart using 21619-1-AP

IHC staining of rat ovary using 21619-1-AP

IHC staining of rat ovary using 21619-1-AP

IF Staining of HeLa using 21619-1-AP

IF Staining of A431 using 21619-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

A signature motif in LIM proteins mediates binding to checkpoint proteins and increases tumour radiosensitivity.

Up-regulated FHL2 inhibits ovulation through interacting with androgen receptor and ERK1/2 in polycystic ovary syndrome.

FHL2 deficiency impairs follicular development and fertility by attenuating EGF/EGFR/YAP signaling in ovarian granulosa cells

Genetic ablation of diabetes-associated gene Ccdc92 reduces obesity and insulin resistance in mice

FHL2 Inhibits SARS-CoV-2 Replication by Enhancing IFN-β Expression through Regulating IRF-3

Structural and Functional Characterization of Host FHL1 Protein Interaction with Hypervariable Domain of Chikungunya Virus nsP3 Protein.