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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FIBP Polyclonal antibody

FIBP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15968-1-AP

Synonyms

FGFIBP, FIBP, FIBP 1

Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using FIBP Polyclonal antibody (15968-1-AP)

    WB analysis of BxPC-3 using 15968-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15968-1-AP (FIBP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using FIBP Polyclonal antibody (15968-1-AP)

    WB analysis of mouse pancreas using 15968-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15968-1-AP (FIBP Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of BxPC-3 cells using FIBP Polyclonal antibody (15968-1-AP)

    WB analysis of BxPC-3 using 15968-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15968-1-AP (FIBP Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using FIBP Polyclonal antibody (15968-1-AP)

    WB analysis of mouse pancreas using 15968-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15968-1-AP (FIBP Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Immunohistochemistry (IHC) staining of human kidney tissue using FIBP Polyclonal antibody (15968-1-AP)

    IHC staining of human kidney using 15968-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15968-1-AP (FIBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using FIBP Polyclonal antibody (15968-1-AP)

    IHC staining of human kidney using 15968-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15968-1-AP (FIBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FIBP Polyclonal antibody (15968-1-AP)

    IF Staining of HepG2 using 15968-1-AP

    Immunofluorescent analysis of HepG2 cells using 15968-1-AP (FIBP antibody) at dilution of 1:25 and Alexa Fluor 594-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inBxPC-3 cells, mouse pancreas tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15968-1-AP targets FIBP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FIBP fusion protein Ag8743 相同性解析による交差性が予測される生物種
Full Name fibroblast growth factor (acidic) intracellular binding protein
Calculated molecular weight 357 aa, 41 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC014388
Gene symbol FIBP
Gene ID (NCBI) 9158
RRIDAB_2878203
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FIBP, also named as Acidic fibroblast growth factor intracellular binding protein, is a 364 amino acid protein, which is highly expressed in heart, skeletal muscle and pancreas. FIBP may be involved in mitogenic function of FGF1.

Protocols

Product Specific Protocols
WB protocol for FIBP antibody 15968-1-APDownload protocol
IHC protocol for FIBP antibody 15968-1-APDownload protocol
IF protocol for FIBP antibody 15968-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols