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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using FIP1L1 Recombinant antibody (83863-3-RR)

    IHC staining of human ovary cancer using 83863-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83863-3-RR (FIP1L1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using FIP1L1 Recombinant antibody (83863-3-RR)

    IF Staining of MCF-7 using 83863-3-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using FIP1L1 antibody (83863-3-RR, Clone: 240658A8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of A549 cells using FIP1L1 Recombinant antibody (83863-3-RR)

    FC experiment of A549 using 83863-3-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug FIP1L1 Recombinant antibody (83863-3-RR, Clone:240658A8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Flow cytometry (FC) experiment of U-2 OS using FIP1L1 Recombinant antibody (83863-3-RR)

    FC experiment of U-2 OS using 83863-3-RR

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug FIP1L1 Recombinant antibody (83863-3-RR, Clone:240658A8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83863-3-RR

    Affinity and Kinetic Characterization of 83863-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83863-3-RR against Human FIP1L1 were performed. The affinity constant is 1.63 nM.

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FIP1L1 Recombinant antibody

FIP1L1 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83863-3-RR
Clone No.240658A8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

240658A8, FIP1, FIP1-like 1 protein, Pre-mRNA 3'-end-processing factor FIP1, RHE

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inA549 cells, U2OS cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83863-3-RR targets FIP1L1 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FIP1L1 fusion protein Ag2921 相同性解析による交差性が予測される生物種
Full Name FIP1 like 1 (S. cerevisiae)
Calculated molecular weight 594 aa, 66 kDa
GenBank accession numberBC017724
Gene Symbol FIP1L1
Gene ID (NCBI) 81608
RRIDAB_3671449
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ6UN15
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FIP1L1, also known as FIP1, encodes a subunit of the CPSF (cleavage and polyadenylation specificity factor) complex that polyadenylates the 3' end of mRNA precursors. The FIP1L1-PDGFRA fusion gene encodes a constitutively activated tyrosine kinase that links the first 233 amino acids of FIP1L1 to the last 523 amino acids of PDGFRA. This gene fusion and chromosomal deletion causes some forms of idiopathic hypereosinophilic syndrome (HES)( PMID: 12660384).

Protocols

Product Specific Protocols
IHC protocol for FIP1L1 antibody 83863-3-RRDownload protocol
IF protocol for FIP1L1 antibody 83863-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols