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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FIS1 Monoclonal antibody (66635-1-Ig)

    WB analysis using 66635-1-Ig (same clone as 66635-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66635-1-Ig (FIS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66635-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FIS1 Monoclonal antibody (66635-1-Ig)

    WB analysis using 66635-1-Ig (same clone as 66635-1-PBS)

    Various cells were subjected to SDS PAGE followed by western blot with 66635-1-Ig (FIS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66635-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using FIS1 Monoclonal antibody (66635-1-Ig)

    IP experiment of HeLa using 66635-1-Ig (same clone as 66635-1-PBS)

    IP result of anti-FIS1 (IP:66635-1-Ig, 4ug; Detection:66635-1-Ig 1:1000) with HeLa cells lysate 1120 ug. This data was developed using the same antibody clone with 66635-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FIS1 Monoclonal antibody (66635-1-Ig)

    IHC staining of human breast cancer using 66635-1-Ig (same clone as 66635-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66635-1-Ig (FIS1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66635-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FIS1 Monoclonal antibody (66635-1-Ig)

    IHC staining of human breast cancer using 66635-1-Ig (same clone as 66635-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66635-1-Ig (FIS1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66635-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using FIS1 Monoclonal antibody (66635-1-Ig)

    IHC staining of human prostate cancer using 66635-1-Ig (same clone as 66635-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66635-1-Ig (FIS1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66635-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using FIS1 Monoclonal antibody (66635-1-Ig)

    IHC staining of human prostate cancer using 66635-1-Ig (same clone as 66635-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66635-1-Ig (FIS1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66635-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using FIS1 Monoclonal antibody (66635-1-Ig)

    IHC staining of human ovary tumor using 66635-1-Ig (same clone as 66635-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66635-1-Ig (FIS1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66635-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using FIS1 Monoclonal antibody (66635-1-Ig)

    IHC staining of human ovary tumor using 66635-1-Ig (same clone as 66635-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66635-1-Ig (FIS1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66635-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FIS1 Monoclonal antibody (66635-1-Ig)

    IF Staining of HepG2 using 66635-1-Ig (same clone as 66635-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using FIS1 antibody (66635-1-Ig, Clone: 2F10E1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66635-1-PBS in a different storage buffer formulation.

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FIS1 Monoclonal antibody, PBS Only

FIS1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66635-1-PBS
Clone No.2F10E1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

TPR repeat protein 11, hFis1, FIS1 homolog, CGI-135, CGI 135

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66635-1-PBS targets FIS1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FIS1 fusion protein Ag1409 相同性解析による交差性が予測される生物種
Full Name fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae)
Calculated molecular weight 17 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC009428
Gene Symbol FIS1
Gene ID (NCBI) 51024
RRIDAB_2881994
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y3D6
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Fis1 (fission 1) is an integral mitochondrial outer membrane protein that participates in mitochondrial fission by interacting with dynamin-related protein 1 (Drp1). Excessive mitochondrial fission is associated with the pathology of a number of neurodegenerative or neurodevelopmental diseases. Increased expression of Fis1 has been found in Huntington's disease (HD)-affected brain, Alzheimer's disease (AD) patients, and autism spectrum disorder. (21257639, 21459773, 23333625)