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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FITC Monoclonal antibody (68132-1-Ig)

    WB analysis using 68132-1-Ig

    WB result of FITC antibody (68132-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with FITC conjugated protein and un-conjugated protein as negative control. Antibody to TDP-43 as the positive control to target protein.

  • Western Blot (WB) analysis of BSA using FITC Monoclonal antibody (68132-1-Ig)

    WB analysis of BSA using 68132-1-Ig

    WB results of FITC antibody (68132-1-Ig, 1:10000) with BSA and FITC conjugated BSA.

  • ELISA experiment of FITC conjugated antibody using FITC Monoclonal antibody (68132-1-Ig)

    ELISA experiment of FITC conjugated antibody using 68132-1-Ig

    Indirect ELISA and competitive ELISA results show that this antibody is specific to FITC. Indirect ELISA was performed by coating FITC conjugated BSA at 0ng/well followed by blocking with 1% BSA. Serial diluted primary antibody was added to the plates and incubated at 37℃. HRP-goat anti-mouse was used for detection (red curve, refer to top X-right Y axis). Competitive ELISA was performed similarly except that different concentration of FITC (active group been quenched) was mixed in 3.3ng/mL primary antibody (blue curve, refere to bottom X-left Y axis).

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FITC Monoclonal antibody

FITC Monoclonal Antibody for WB, ELISA
Cat No. 68132-1-Ig
Clone No.3C10A1
Publications(1)

Host / Isotype

Mouse / IgG2a

Reactivity

chemical compound, fitc and More (1)

Applications

WB, ELISA

Synonyms

Fluorescein isothiocyanate

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 1000 ug/ml
20 μL, 1000 ug/ml
150 μL, 1000 ug/ml

¥67,000/ 150 μL

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inFITC-TDP43, FITC-Ag1231, BSA
Positive ELISA detected inFITC conjugated antibody

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Enzyme-linked Immunosorbent Assay (ELISA)ELISA :
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

68132-1-Ig targets FITC in WB, ELISA applications and shows reactivity with chemical compound, fitc samples.

Tested Reactivity chemical compound, fitc
Cited Reactivitymouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Compound 相同性解析による交差性が予測される生物種
Full Name FITC
Gene Symbol
Gene ID (NCBI)
RRIDAB_2923659
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications. It is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. It is typically available as a mixture of isomers, fluorescein 5-isothiocyanate (5-FITC) and fluorescein 6-isothiocyanate (6-FITC). FITC is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. CAS number of FITC: 27072-45-3 (mixture); 3326-32-7, 5-isomer; 18861-78-4 (6-isomer). This mouse monoclonal antibody detects the FITC only. This antibody can be coated in the plate to capture the FITC conjugated protein or antibody.

Protocols

Product Specific Protocols
WB protocol for FITC antibody 68132-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Environ Health Perspect

Organophosphorus Flame Retardants and Metabolic Disruption: An in Silico, in Vitro, and in Vivo Study Focusing on Adiponectin Receptors

Authors - Ying Liu
View Article