Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | rat brain tissue |
| Positive IHC detected in | mouse brain tissue, human liver tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
Product Information
17417-1-AP targets FJX1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FJX1 fusion protein Ag11385 相同性解析による交差性が予測される生物種 |
| Full Name | four jointed box 1 (Drosophila) |
| Calculated molecular weight | 437 aa, 49 kDa |
| Observed molecular weight | 49 kDa |
| GenBank accession number | BC049171 |
| Gene Symbol | FJX1 |
| Gene ID (NCBI) | 24147 |
| RRID | AB_2878406 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86VR8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Four-jointed box protein 1 (FJX1), also named as FLJ22416, is a 437 amino acid protein, which belongs to the FJX1 family. FJX1 is a secreted protein and acts as an inhibitor of dendrite and branching. FJX1 is important for growth and differentiation of legs and wings, and for proper development of the eyes. FAT4, an essential gene that has a key role in vertebrate PCP (planar cell polarity), represses FJX1 expression which may lead to cystic diseases in humans.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FJX1 antibody 17417-1-AP | Download protocol |
| IHC protocol for FJX1 antibody 17417-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Endocrinol Regulation of Sertoli cell function by planar cell polarity (PCP) protein Fjx1 | ||
Reprod Biol Endocrinol Map-1a regulates Sertoli cell BTB dynamics through the cytoskeletal organization of microtubule and F-actin | ||