Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 12172-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 12172-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12172-1-AP
WB result of FKBP10/FKBP65 antibody (12172-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FKBP10/FKBP65 transfected HeLa cells.
WB analysis of human brain using 12172-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 12172-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 12172-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of fetal human brain using 12172-1-AP
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12172-1-AP (FKBP65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 12172-1-AP
IP result of anti-FKBP10/FKBP65 (IP:12172-1-AP, 4ug; Detection:12172-1-AP 1:600) with HEK-293 cells lysate 2800ug.
IHC staining of human ovary cancer using 12172-1-AP
Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 12172-1-AP (FKBP10/FKBP65 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary cancer using 12172-1-AP
Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 12172-1-AP (FKBP10/FKBP65 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

FKBP10/FKBP65 Polyclonal antibody

FKBP10/FKBP65 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 12172-1-AP

Publications(21)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

FKBP10, FKBP65, FKBP 65, FKBP 10, FK506-binding protein 10

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (21)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, human brain tissue, human placenta tissue, human kidney tissue, fetal human brain tissue, HeLa cells, NIH/3T3 cells
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human ovary cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 19 publications below
IHC	See 3 publications below
IF	See 7 publications below
IP	See 1 publications below

Product Information

12172-1-AP targets FKBP10/FKBP65 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, chicken
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	FKBP10/FKBP65 fusion protein Ag2814 相同性解析による交差性が予測される生物種
Full Name	FK506 binding protein 10, 65 kDa
Calculated molecular weight	582 aa, 64 kDa
Observed molecular weight	64 kDa
GenBank accession number	BC016467
Gene Symbol	FKBP10
Gene ID (NCBI)	60681
RRID	AB_2102550
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96AY3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

FKBP10, also known as FKBP65, is a 65 kDa FK506 binding protein that is a member of the FKBP-type peptidyl-prolyl cis/trans isomerase family. This protein localizes to the endoplasmic reticulum and acts as a molecular chaperone and binding partner of type I collagen. FKBP65 is expressed in developing tissues and re-expressed in adult tissues following injury. As documented, FKBP10 has been detected to play important roles in the initiation and progression of cancers.

Protocols

Product Specific Protocols
WB protocol for FKBP10/FKBP65 antibody 12172-1-AP	Download protocol
IHC protocol for FKBP10/FKBP65 antibody 12172-1-AP	Download protocol
IP protocol for FKBP10/FKBP65 antibody 12172-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Mol Cell Proteomic mapping of the human mitochondrial intermembrane space in live cells via ratiometric APEX tagging. Authors - Victoria Hung View Article
human,mouse	WB	Sci Adv Melanoma-intrinsic NR2F6 activity regulates antitumor immunity Authors - Hyungsoo Kim KD Validated View Article
human	WB,IF	Proc Natl Acad Sci U S A Disentangling mechanisms involved in collagen pyridinoline cross-linking: The immunophilin FKBP65 is critical for dimerization of lysyl hydroxylase 2. Authors - Rutger A F Gjaltema KD Validated View Article
mouse	IHC	Proc Natl Acad Sci U S A Localized chondro-ossification underlies joint dysfunction and motor deficits in the Fkbp10 mouse model of osteogenesis imperfecta. Authors - Joohyun Lim View Article
human	WB,IP,IHC,IF	Int J Biol Sci FKBP10 Promotes the Muscle Invasion of Bladder Cancer via Lamin A Dysregulation Authors - Xupeng Zhao View Article
mouse	WB,IF	Hum Mol Genet Connective Tissue Alterations in Fkbp10-/- mice. Authors - Caressa D Lietman View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 12172-1-AP

WB analysis using 12172-1-AP

WB analysis of HeLa using 12172-1-AP

WB analysis of human brain using 12172-1-AP

WB analysis of human placenta using 12172-1-AP

WB analysis of human kidney using 12172-1-AP

WB analysis of fetal human brain using 12172-1-AP

IP experiment of HEK-293 using 12172-1-AP

IHC staining of human ovary cancer using 12172-1-AP

IHC staining of human ovary cancer using 12172-1-AP

FKBP10/FKBP65 Polyclonal antibody

FKBP10/FKBP65 Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Proteomic mapping of the human mitochondrial intermembrane space in live cells via ratiometric APEX tagging.

Melanoma-intrinsic NR2F6 activity regulates antitumor immunity

Disentangling mechanisms involved in collagen pyridinoline cross-linking: The immunophilin FKBP65 is critical for dimerization of lysyl hydroxylase 2.

Localized chondro-ossification underlies joint dysfunction and motor deficits in the Fkbp10 mouse model of osteogenesis imperfecta.

FKBP10 Promotes the Muscle Invasion of Bladder Cancer via Lamin A Dysregulation

Connective Tissue Alterations in Fkbp10-/- mice.