☆ 3/31(月)まで!二次抗体 & FlexAbleが30%オフ! >> 免疫アッセイサポートキャンペーン

  • Featured Product
  • KD/KO Validated

FKBP10/FKBP65 Polyclonal antibody

FKBP10/FKBP65 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 12172-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, IP, ELISA

FKBP10, FKBP65, FKBP 65, FKBP 10, FK506-binding protein 10

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。


国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHEK-293 cells, human brain tissue, human placenta tissue, human kidney tissue, fetal human brain tissue, HeLa cells, NIH/3T3 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12172-1-AP targets FKBP10/FKBP65 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FKBP10/FKBP65 fusion protein Ag2814 相同性解析による交差性が予測される生物種
Full Name FK506 binding protein 10, 65 kDa
Calculated molecular weight 582 aa, 64 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC016467
Gene Symbol FKBP10
Gene ID (NCBI) 60681
RRIDAB_2102550
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96AY3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FKBP10, also known as FKBP65, is a 65 kDa FK506 binding protein that is a member of the FKBP-type peptidyl-prolyl cis/trans isomerase family. This protein localizes to the endoplasmic reticulum and acts as a molecular chaperone and binding partner of type I collagen. FKBP65 is expressed in developing tissues and re-expressed in adult tissues following injury. As documented, FKBP10 has been detected to play important roles in the initiation and progression of cancers.

Protocols

Product Specific Protocols
WB protocol for FKBP10/FKBP65 antibody 12172-1-APDownload protocol
IHC protocol for FKBP10/FKBP65 antibody 12172-1-APDownload protocol
IP protocol for FKBP10/FKBP65 antibody 12172-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell

Proteomic mapping of the human mitochondrial intermembrane space in live cells via ratiometric APEX tagging.

Authors - Victoria Hung
human,mouseWB

Sci Adv

Melanoma-intrinsic NR2F6 activity regulates antitumor immunity

Authors - Hyungsoo Kim
  • KD Validated
humanWB,IF

Proc Natl Acad Sci U S A

Disentangling mechanisms involved in collagen pyridinoline cross-linking: The immunophilin FKBP65 is critical for dimerization of lysyl hydroxylase 2.

Authors - Rutger A F Gjaltema
  • KD Validated
mouseIHC

Proc Natl Acad Sci U S A

Localized chondro-ossification underlies joint dysfunction and motor deficits in the Fkbp10 mouse model of osteogenesis imperfecta.

Authors - Joohyun Lim
humanWB,IP,IHC,IF

Int J Biol Sci

FKBP10 Promotes the Muscle Invasion of Bladder Cancer via Lamin A Dysregulation

Authors - Xupeng Zhao
mouseWB,IF

Hum Mol Genet

Connective Tissue Alterations in Fkbp10-/- mice.

Authors - Caressa D Lietman
Loading...