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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FKBP1B Polyclonal antibody

FKBP1B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15114-1-AP

Synonyms

12.6 kDa FK506 binding protein, 12.6 kDa FKBP, FK506 binding protein 1B, FKBP 12.6, FKBP 1B, FKBP12.6, FKBP1B, FKBP1L, FKBP9, h FKBP 12, Immunophilin FKBP12.6, OTK4, PKBP1L, PPIase, PPIase FKBP1B, Rotamase

Validation Data Gallery

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  • Western Blot (WB) analysis of U-937 cells using FKBP1B Polyclonal antibody (15114-1-AP)

    WB analysis of U-937 using 15114-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 15114-1-AP (FKBP1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using FKBP1B Polyclonal antibody (15114-1-AP)

    WB analysis of mouse brain using 15114-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15114-1-AP (FKBP1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using FKBP1B Polyclonal antibody (15114-1-AP)

    WB analysis of mouse brain using 15114-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15114-1-AP (FKBP1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using FKBP1B Polyclonal antibody (15114-1-AP)

    WB analysis of mouse heart using 15114-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 15114-1-AP (FKBP1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using FKBP1B Polyclonal antibody (15114-1-AP)

    WB analysis of Jurkat using 15114-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15114-1-AP (FKBP1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using FKBP1B Polyclonal antibody (15114-1-AP)

    WB analysis of U-937 using 15114-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 15114-1-AP (FKBP1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using FKBP1B Polyclonal antibody (15114-1-AP)

    IHC staining of human heart using 15114-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15114-1-AP (FKBP1B antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using FKBP1B Polyclonal antibody (15114-1-AP)

    IHC staining of human heart using 15114-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15114-1-AP (FKBP1B antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inU-937 cells, mouse brain tissue, mouse heart tissue, Jurkat cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

15114-1-AP targets FKBP1B in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FKBP1B fusion protein Ag7153 相同性解析による交差性が予測される生物種
Full Name FK506 binding protein 1B, 12.6 kDa
Calculated molecular weight 12 kDa
Observed molecular weight 12 kDa
GenBank accession numberBC002614
Gene symbol FKBP1B
Gene ID (NCBI) 2281
RRIDAB_11182817
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FKBP1B antibody 15114-1-APDownload protocol
IHC protocol for FKBP1B antibody 15114-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Neuron

Single-nucleus RNA sequencing demonstrates an autosomal dominant Alzheimer's disease profile and possible mechanisms of disease protection

Authors - Maria Camila Almeida
View Article