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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse cerebellum tissue using FKBP2 Polyclonal antibody (11700-1-AP)

    WB analysis of mouse cerebellum using 11700-1-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 11700-1-AP (FKBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using FKBP2 Polyclonal antibody (11700-1-AP)

    WB analysis of HeLa using 11700-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11700-1-AP (FKBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using FKBP2 Polyclonal antibody (11700-1-AP)

    WB analysis of MCF-7 using 11700-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 11700-1-AP (FKBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using FKBP2 Polyclonal antibody (11700-1-AP)

    IHC staining of human prostate cancer using 11700-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11700-1-AP (FKBP2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using FKBP2 Polyclonal antibody (11700-1-AP)

    IHC staining of human prostate cancer using 11700-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11700-1-AP (FKBP2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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FKBP2 Polyclonal antibody

FKBP2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11700-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

FKBP13, FKBP 2, FKBP 13, FK506-binding protein 2, EC:5.2.1.8

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse cerebellum tissue, HeLa cells, MCF-7 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

11700-1-AP targets FKBP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FKBP2 fusion protein Ag2255 相同性解析による交差性が予測される生物種
Full Name FK506 binding protein 2, 13kDa
Calculated molecular weight 142 aa, 16 kDa
Observed molecular weight 13-16 kDa
GenBank accession numberBC003384
Gene Symbol FKBP2
Gene ID (NCBI) 2286
RRIDAB_2102876
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP26885
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FKBP2 is also named as FKBP13 and belongs to the FKBP-type PPIase family. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. FKBP2 has a 21-amino acid signal peptide and appears to be membrane-associated(PMID:1713687). It is localized to the lumen of the endoplasmic reticulum (ER). FKBP12 and FKBP13 are highly similar proteins, of molecular masses 12 kDa and 13 kDa respectively, with approx.43 % amino acid identity. The strong homology between FKBP12 and FKBP13 suggests that they may share similar biological functions, although, apart from rotamase activity, details relating to the function of either protein are scant(PMID:8373365).

Protocols

Product Specific Protocols
WB protocol for FKBP2 antibody 11700-1-APDownload protocol
IHC protocol for FKBP2 antibody 11700-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Physiol

Multiple roles for peptidylglycine α-amidating monooxygenase in the response to hypoxia.

Authors - Vishwanatha K S Rao
View Article
mouseWB

Endocrinology

Changes in Corticotrope Gene Expression Upon Increased Expression of Peptidylglycine α-Amidating Monooxygenase.

Authors - Richard E Mains
View Article