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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FKBP2 Recombinant antibody (84077-6-RR)

    WB analysis using 84077-6-RR (same clone as 84077-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84077-6-RR (FKBP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84077-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using FKBP2 Recombinant antibody (84077-6-RR)

    IHC staining of human placenta using 84077-6-RR (same clone as 84077-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84077-6-RR (FKBP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84077-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FKBP2 Recombinant antibody (84077-6-RR)

    IHC staining of mouse brain using 84077-6-RR (same clone as 84077-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84077-6-RR (FKBP2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84077-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84077-6-RR

    Affinity and Kinetic Characterization of 84077-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84077-6-RR against Human FKBP2 were performed. The affinity constant is below 1 pM.

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FKBP2 Recombinant antibody, PBS Only

FKBP2 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84077-6-PBS
Clone No.241166B9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

FKBP 2, FKBP 13, FK506-binding protein 2, EC:5.2.1.8, 241166B9

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84077-6-PBS targets FKBP2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FKBP2 fusion protein Ag2255 相同性解析による交差性が予測される生物種
Full Name FK506 binding protein 2, 13kDa
Calculated molecular weight 142 aa, 16 kDa
Observed molecular weight 13-16 kDa
GenBank accession numberBC003384
Gene Symbol FKBP2
Gene ID (NCBI) 2286
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP26885
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

FKBP2 is also named as FKBP13 and belongs to the FKBP-type PPIase family. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. FKBP2 has a 21-amino acid signal peptide and appears to be membrane-associated(PMID:1713687). It is localized to the lumen of the endoplasmic reticulum (ER). FKBP12 and FKBP13 are highly similar proteins, of molecular masses 12 kDa and 13 kDa respectively, with approx.43 % amino acid identity. The strong homology between FKBP12 and FKBP13 suggests that they may share similar biological functions, although, apart from rotamase activity, details relating to the function of either protein are scant (PMID:8373365).