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  • Featured Product
  • KD/KO Validated

FKBP3 Polyclonal antibody

FKBP3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11755-1-AP

Synonyms

FKBP25, FKBP 3, FKBP 25, FK506-binding protein 3, EC:5.2.1.8

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using FKBP3 Polyclonal antibody (11755-1-AP)

    WB analysis of HeLa using 11755-1-AP

    WB result of FKBP3 antibody (11755-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FKBP3 transfected HeLa cells.

  • Western Blot (WB) analysis of PC-3 cells using FKBP3 Polyclonal antibody (11755-1-AP)

    WB analysis of PC-3 using 11755-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 11755-1-AP (FKBP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using FKBP3 Polyclonal antibody (11755-1-AP)

    WB analysis of HeLa using 11755-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11755-1-AP (FKBP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using FKBP3 Polyclonal antibody (11755-1-AP)

    WB analysis of PC-3 using 11755-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 11755-1-AP (FKBP3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using FKBP3 Polyclonal antibody (11755-1-AP)

    WB analysis of A431 using 11755-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 11755-1-AP (FKBP3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FKBP3 Polyclonal antibody (11755-1-AP)

    IHC staining of mouse brain using 11755-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FKBP3 Polyclonal antibody (11755-1-AP)

    IHC staining of mouse brain using 11755-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using FKBP3 Polyclonal antibody (11755-1-AP)

    IHC staining of human testis using 11755-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using FKBP3 Polyclonal antibody (11755-1-AP)

    IHC staining of human testis using 11755-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using FKBP3 Polyclonal antibody (11755-1-AP)

    IHC staining of human lung cancer using 11755-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using FKBP3 Polyclonal antibody (11755-1-AP)

    IHC staining of human lung cancer using 11755-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11755-1-AP (FKBP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FKBP3 Polyclonal antibody (11755-1-AP)

    IF Staining of HeLa using 11755-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FKBP3 antibody (11755-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inPC-3 cells, HeLa cells, A431 cells
Positive IHC detected inmouse brain tissue, human lung cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11755-1-AP targets FKBP3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FKBP3 fusion protein Ag2351 相同性解析による交差性が予測される生物種
Full Name FK506 binding protein 3, 25kDa
Calculated molecular weight 224 aa, 25 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC020809
Gene symbol FKBP3
Gene ID (NCBI) 2287
RRIDAB_2102983
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FKBP3 antibody 11755-1-APDownload protocol
IHC protocol for FKBP3 antibody 11755-1-APDownload protocol
IF protocol for FKBP3 antibody 11755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Mol Med

FKBP3 aggravates the malignant phenotype of diffuse large B-cell lymphoma by PARK7-mediated activation of Wnt/β-catenin signalling

Authors - Xiaojing Xing
  • KD Validated
View Article
humanIHC

BMC Cancer

Construction of the optimization prognostic model based on differentially expressed immune genes of lung adenocarcinoma.

Authors - Yang Zhai
View Article