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KD/KO Validated

FKBP52 Polyclonal antibody

Name: FKBP52 Polyclonal antibody
Brand: Proteintech
SKU: 10655-1-AP

FKBP52 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10655-1-AP

Synonyms

FKBP4, FKBP-4, FK506-binding protein 4, EC:5.2.1.8, 52 kDa FK506-binding protein

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 10655-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10655-1-AP (FKBP52 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 10655-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10655-1-AP (FKBP52 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 10655-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 10655-1-AP (FKBP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 10655-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10655-1-AP (FKBP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 10655-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 10655-1-AP (FKBP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 10655-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 10655-1-AP (FKBP52 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 10655-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10655-1-AP (FKBP52 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10655-1-AP
IP result of anti-FKBP52 (IP:10655-1-AP, 4ug; Detection:10655-1-AP 1:20000) with HeLa cells lysate 1360 ug.
IHC staining of human prostate cancer using 10655-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10655-1-AP (FKBP52 antibody) at dilution of 1:100 (under 25x lens).
IF Staining of MCF-7 using 10655-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using FKBP52 antibody (10655-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 10655-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, HSC-T6 cells, Jurkat cells, NIH/3T3 cells, PC-3 cells, MCF-7 cells, C6 cells, rat testis tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 15 publications below
IHC	See 4 publications below
IP	See 1 publications below

Product Information

10655-1-AP targets FKBP52 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, chicken
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	FKBP52 fusion protein Ag1019 相同性解析による交差性が予測される生物種
Full Name	FK506 binding protein 4, 59kDa
Calculated molecular weight	52 kDa
Observed molecular weight	52 kDa
GenBank accession number	BC007924
Gene symbol	FKBP52
Gene ID (NCBI)	2288
RRID	AB_2246853
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

FKBP52 (FK506 binding protein 4) is a member of the immunophilin protein family that comprises intracellular protein effectors of immunosuppressive drugs, and it is known as a steroid receptor-associated protein. As a novel regulator of microtubule dynamics, FKBP52 is associated with the motor protein dynein and with the cytoskeleton during mitosis. FKBP52 is highly expressed in CNS regions susceptible to Alzheimer's, and plays a role in modulating toxicity of Abeta peptides. The protein can associate with the Tau function, and may help to decipher and modulate the events involved in Tau-induced neurodegeneration. In addition, FKBP52 is likely to play a role in growth and development of the male genitalia.

Protocols

Product Specific Protocols
WB protocol for FKBP52 antibody 10655-1-AP	Download protocol
IHC protocol for FKBP52 antibody 10655-1-AP	Download protocol
IF protocol for FKBP52 antibody 10655-1-AP	Download protocol
IP protocol for FKBP52 antibody 10655-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Theranostics FKBP4 connects mTORC2 and PI3K to activate the PDK1/Akt-dependent cell proliferation signaling in breast cancer. Authors - Alain Mangé KD Validated View Article
human	WB,IHC	Clin Cancer Res Identification of a new panel of serum autoantibodies associated with the presence of in situ carcinoma of the breast in younger women. Authors - Desmetz Caroline C View Article
human	WB，IP	Mol Oncol FKBP51 and FKBP52 regulate androgen receptor dimerization and proliferation in prostate cancer cells. Authors - Keisuke Maeda KD Validated View Article
human	WB	Int J Mol Sci Secretome Analyses Identify FKBP4 as a GBA1-Associated Protein in CSF and iPS Cells from Parkinson's Disease Patients with GBA1 Mutations Authors - Rika Kojima View Article
human	WB	J Cell Physiol Multiple roles for peptidylglycine α-amidating monooxygenase in the response to hypoxia. Authors - Vishwanatha K S Rao View Article
mouse	WB	RNA The co-chaperones Fkbp4/5 control Argonaute2 expression and facilitate RISC assembly. Authors - Martinez Natalia J NJ KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FKBP52 Polyclonal antibody

FKBP52 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 10655-1-AP

WB analysis using 10655-1-AP

WB analysis of PC-3 using 10655-1-AP

WB analysis of Jurkat using 10655-1-AP

WB analysis of PC-3 using 10655-1-AP

WB analysis of PC-3 using 10655-1-AP

WB analysis of NIH/3T3 using 10655-1-AP

IP experiment of HeLa using 10655-1-AP

IHC staining of human prostate cancer using 10655-1-AP

IF Staining of MCF-7 using 10655-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

FKBP4 connects mTORC2 and PI3K to activate the PDK1/Akt-dependent cell proliferation signaling in breast cancer.

Identification of a new panel of serum autoantibodies associated with the presence of in situ carcinoma of the breast in younger women.

FKBP51 and FKBP52 regulate androgen receptor dimerization and proliferation in prostate cancer cells.

Secretome Analyses Identify FKBP4 as a GBA1-Associated Protein in CSF and iPS Cells from Parkinson's Disease Patients with GBA1 Mutations

Multiple roles for peptidylglycine α-amidating monooxygenase in the response to hypoxia.

The co-chaperones Fkbp4/5 control Argonaute2 expression and facilitate RISC assembly.