• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using FKBP52 Monoclonal antibody (66040-1-Ig)

    WB analysis of HeLa using 66040-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66040-1-Ig (FKBP52 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using FKBP52 Monoclonal antibody (66040-1-Ig)

    WB analysis of K-562 using 66040-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 66040-1-Ig (FKBP52 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using FKBP52 Monoclonal antibody (66040-1-Ig)

    WB analysis of Jurkat using 66040-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66040-1-Ig (FKBP52 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using FKBP52 Monoclonal antibody (66040-1-Ig)

    WB analysis of HeLa using 66040-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66040-1-Ig (FKBP52 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using FKBP52 Monoclonal antibody (66040-1-Ig)

    WB analysis of MCF-7 using 66040-1-Ig

    MCF7 cells were subjected to SDS PAGE followed by western blot with 66040-1-Ig (FKBP52 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using FKBP52 Monoclonal antibody (66040-1-Ig)

    IHC staining of human cervical cancer using 66040-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66040-1-Ig (FKBP52 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using FKBP52 Monoclonal antibody (66040-1-Ig)

    IHC staining of human cervical cancer using 66040-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66040-1-Ig (FKBP52 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FKBP52 Monoclonal antibody (66040-1-Ig)

    IHC staining of human colon cancer using 66040-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66040-1-Ig (FKBP52 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using FKBP52 Monoclonal antibody (66040-1-Ig)

    IHC staining of human colon cancer using 66040-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66040-1-Ig (FKBP52 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FKBP52 Monoclonal antibody (66040-1-Ig)

    IF Staining of HeLa using 66040-1-Ig

    Immunofluorescent analysis of HeLa cells using 66040-1-Ig (FKBP52 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

☆ 3/31（月）まで！二次抗体 & FlexAbleが30%オフ！ >> 免疫アッセイサポートキャンペーン

FKBP52 Monoclonal antibody

FKBP52 Monoclonal Antibody for WB, IF, IHC, ELISA
Cat No. 66040-1-Ig
Clone No.3F5F9

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, IHC, ELISA

Synonyms

51 kDa FK506 binding protein, 52 kDa FK506 binding protein, 52 kDa FKBP, 59 kDa immunophilin, FK506 binding protein 4, FK506 binding protein 4, 59kDa, FKBP 4, FKBP 52, FKBP4, FKBP51, FKBP52, FKBP59, HBI, HSP binding immunophilin, Hsp56, Immunophilin FKBP52, p52, p59, PPIase, PPIase FKBP4, Rotamase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, MCF-7 cells, K-562 cells
Positive IHC detected inhuman cervical cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66040-1-Ig targets FKBP52 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FKBP52 fusion protein Ag18704 相同性解析による交差性が予測される生物種
Full Name FK506 binding protein 4, 59kDa
Calculated molecular weight 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC007924
Gene Symbol FKBP52
Gene ID (NCBI) 2288
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ02790
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FKBP52 (FK506 binding protein 4) is a member of the immunophilin protein family that comprises intracellular protein effectors of immunosuppressive drugs, and it is known as a steroid receptor-associated protein. As a novel regulator of microtubule dynamics, FKBP52 is associated with the motor protein dynein and with the cytoskeleton during mitosis. FKBP52 is highly expressed in CNS regions susceptible to Alzheimer's, and plays a role in modulating toxicity of Abeta peptides. The protein can associate with the Tau function, and may help to decipher and modulate the events involved in Tau-induced neurodegeneration. In addition, FKBP52 is likely to play a role in growth and development of the male genitalia.

Protocols

Product Specific Protocols
WB protocol for FKBP52 antibody 66040-1-IgDownload protocol
IHC protocol for FKBP52 antibody 66040-1-IgDownload protocol
IF protocol for FKBP52 antibody 66040-1-IgDownload protocol
FC protocol for FKBP52 antibody 66040-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols