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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FKBP52 Monoclonal antibody, PBS Only

FKBP52 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B5F1

Cat no : 66040-2-PBS

Synonyms

51 kDa FK506 binding protein, 52 kDa FK506 binding protein, 52 kDa FKBP, 59 kDa immunophilin, FK506 binding protein 4, FK506 binding protein 4, 59kDa, FKBP 4, FKBP 52, FKBP4, FKBP51, FKBP52, FKBP59, HBI, HSP binding immunophilin, Hsp56, Immunophilin FKBP52, p52, p59, PPIase, PPIase FKBP4, Rotamase

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FKBP52 Monoclonal antibody (66040-2-Ig)

    WB analysis using 66040-2-Ig (same clone as 66040-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66040-2-Ig (FKBP52 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66040-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using FKBP52 Monoclonal antibody (66040-2-Ig)

    WB analysis of HSC-T6 using 66040-2-Ig (same clone as 66040-2-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66040-2-Ig (FKBP52 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66040-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FKBP52 Monoclonal antibody (66040-2-Ig)

    IHC staining of human breast cancer using 66040-2-Ig (same clone as 66040-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66040-2-Ig (FKBP52 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66040-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FKBP52 Monoclonal antibody (66040-2-Ig)

    IHC staining of human breast cancer using 66040-2-Ig (same clone as 66040-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66040-2-Ig (FKBP52 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66040-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using FKBP52 Monoclonal antibody (66040-2-Ig)

    IF Staining of human breast cancer using 66040-2-Ig (same clone as 66040-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using FKBP52 antibody (66040-2-Ig, Clone: 1B5F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66040-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using FKBP52 Monoclonal antibody (66040-2-Ig)

    IF Staining of human breast cancer using 66040-2-Ig (same clone as 66040-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using FKBP52 antibody (66040-2-Ig, Clone: 1B5F1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66040-2-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66040-2-PBS targets FKBP52 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FKBP52 fusion protein Ag18704 相同性解析による交差性が予測される生物種
Full Name FK506 binding protein 4, 59kDa
Calculated molecular weight 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC007924
Gene symbol FKBP4
Gene ID (NCBI) 2288
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

FKBP52 (FK506 binding protein 4) is a member of the immunophilin protein family that comprises intracellular protein effectors of immunosuppressive drugs, and it is known as a steroid receptor-associated protein. As a novel regulator of microtubule dynamics, FKBP52 is associated with the motor protein dynein and with the cytoskeleton during mitosis. FKBP52 is highly expressed in CNS regions susceptible to Alzheimer's, and plays a role in modulating toxicity of Abeta peptides. The protein can associate with the Tau function, and may help to decipher and modulate the events involved in Tau-induced neurodegeneration. In addition, FKBP52 is likely to play a role in growth and development of the male genitalia.